For climacteric fruits, such as mango, losses have been reported from harvest to fruit consumption. Thus, controlled refrigeration and passive modified atmosphere have been used in combination with edible and biodegradable coatings or plastic packaging to minimise these losses. The present study aimed to evaluate the influence of carnauba wax and Xtend plastic packaging film, with and without refrigerated storage, on the post-harvest preservation of 'Tommy Atkins' mangoes. The fruits were coated with carnauba wax and/or wrapped in Xtend bags and then stored at 12 ± 1 and 25 ± 3 °C for 21 days. The weight loss, fruit firmness, total soluble solids, titratable acidity, vitamin C content, and the CO2 concentration inside the package were determined at 7-day intervals. Cold storage was observed to slow down the rate of degradation and to reduce adverse degradation reactions. By coating the fruits with carnauba wax, packaging them with Xtend plastic and keeping them under refrigeration, it was possible to store the mangoes at 12 ± 1 °C for 21 days. In this way, the CO2 concentration was reduced, mass loss and acidity were decreased, and the fruit firmness was preserved.

Enzyme activity in solid-state fermentation complex added to diets before and after extrusion process and its influence on Nile tilapia (Oreochromis niloticus) performance were evaluated. The following enzymes were analyzed: b-glucosidase, pectinase, xylanase, endoglucanase, amylase, protease, and phytase. The fish were fed five experimental diets: a control diet (without enzyme), two test diets supplemented before extrusion (400 and 800 ppm SSF), and two test diets after extrusion or “on top” (400 and 800 ppm SSF). The experiment had a completely randomized design with four replicates and seven fish per tank. Average initial weight was 10.37 ± 0.95 g, and fish were fed four times a day to satiation for 56 days. The following performance parameters were evaluated: final weight, weight gain, feed conversion, survival, specific growth rate, and body composition. The results were compared using ANOVA and Tukey’s test at p<0.05. All the enzymes showed partial or total losses of activity when subjected to the extrusion process, except phytase. No differences were observed for survival, water quality parameters, and body composition. On-top inclusion provided a better performance, which is observed through increases in final weight, weight gain, and feed conversion. As a result, 800 ppm of enzyme complex SSF should be added "on top", that is after extrusion, to improve tilapia performance and, consequently, contribute to the fish farming financial efficiency.

Studies on the use of agricultural residues as an alternative to commercial substrates have become fundamental tominimising the risk of environmental contamination resulting from inadequate disposal, as well as reducing seedling production costs. This research involved an evaluation of growth variables and quality of cucumber seedlings produced in substrates with different compositions of agricultural waste with a view to providing substitutes for commercial substrate. The experiment was based on a completely randomised design, with six treatments and ten replicates. There were five treatments with increasing proportions of moinha/decreasing proportions of carbonized rice husk (0/40, 10/30, 20/20, 30/10 and 40/0%) and fixed proportions of coconut fibre (15%), eggshell (5%), pine bark (40%), and one commercial substrate treatment as the control (Bioplant®). The variables evaluated were: electrical conductivity of the substrate, plant height, stem diameter, number of leaves, Dickson quality index (DQI) and dry matter of roots and above ground parts. Results show that alternative substrates can replace commercial substrate without impairing the quality of cucumber seedlings. However, the substrate containing 40% of moinha, 0% of rice husk, 15% of coconut fibre, 5% of eggshell and 40% of pine bark resulted in the highest values for all evaluated variables and appears to be the most promising alternative substrate for the production of cucumber seedlings.

Agronomic responses to the use of agricultural inputs have been used in agriculture to identify the optimal level of nutrients to be supplied for high yield. This strategy helps to avoid economic losses and excessive application of fertilizers that has caused pollution of aquifers and soil degradation. The aim of the study was to evaluate water use efficiency, agronomic efficiency of nitrogen, heat units and use of evapotranspiration in yield and its components in sunflowers under hot climate conditions in function of nitrogen fertilizer application during two sowing season. The experiment was conducted in the field. The treatments were two sowing seasons and three levels of nitrogen fertilizer application and one control: 0, 60, 80 and 120 kg N ha-1. The nitrogen source was urea. The experimental design was complete random blocks with factorial array and four replications. The occurrence of cv. Cobalto sunflower phenological stages were not modified by the treatments. Urea favored agronomic efficiency and water use efficiency. Yield components were modified positively, with increasing receptacle area, 100-grain weight, and grain yield per receptacle. Application of nitrogen fertilizer favored sunflower development, although environmental conditions are of critical importance as demonstrated by a clear interaction between the yield response to fertilizer application and the environment where the plants grew.

Nitisols are livelihood sources of agricultural and socio-economic development in several underdeveloped countries worldwide, including Brazil. Brazilian Nitisols are usually associated with basaltic rocks found in the Southern and Southeastern regions of the country. However, Nitisols derived from basalt also occur on the southern coast of Pernambuco state, a region under full urban, agricultural, and industrial development. Aiming to understand the relationship between climate and attributes of Nitisols from the southern coast of Pernambuco, we performed morphological descriptions, physical and chemical analyzes, iron selective extraction, and soil mineralogy (sand, silt, and clay) for three basaltic soil profiles along a land slope. The results suggest a strong weathering of the profiles due to climate conditions, besides a great loss of basic cations and xanthization process, resulting in the natural genesis of acric Nitisols with yellowish coloration, which has not been yet cataloged for this soil order in the Brazilian taxonomic system. In addition, there is a variation for physical attributes of Nitisols along the slope.

The objective of this study was to evaluate genetic divergence among cowpea genotypes and to select parents for crosses aimed at the fresh pod and grain market. Two experiments were carried out during 2014, corresponding to two sowing times, in the municipality of Mossoró, State of Rio Grande do Norte. Twenty-three cowpea genotypes were evaluated in a randomized complete block design with four replicates. Fifteen descriptors were used to quantify divergence, using the Mahalanobis distance as a measure of dissimilarity, obtained from the genotypic mean predicted by the Restricted Maximum Likelihood/Best Linear Unbiased Prediction (REML/BLUP) method. The Unweighted Pair Group Method with Arithmetic Mean (UPGMA) hierarchical method was used to group genotypes and the Singh criterion to quantify the contribution of traits to genetic divergence. The genotype × environment interaction (G × E) influenced divergence, both in the contribution of traits and in the grouping of genotypes. In the experiment 1, the genotypes were distributed among four groups. In the experiment 2, less discrimination occurred and the genotypes were represented by only two groups. When joint analysis of two evaluations was carried out based on two sowing times, genotypes were represented by six groups. The G × E interaction influences the contribution of traits and the grouping of cowpea genotypes in the study of divergence. The genetic divergence of the set of cowpea genotypes evaluated is mainly due to green grain and pod yield. BRS Aracê and BRS Xiquexique cultivars are the most divergent among the genotypes studied, representing 75% of the recommended crosses.

This study aimed to establish an adequate tetrazolium test procedure to estimate okra seed viability. Initially, seeds from four okra lots were immersed in alcohol (98%) for 60 minutes, followed by an 18-h soak in distilled water at 25 °C. Then, the seeds were longitudinally sectioned, immersed in 0.025, 0.050 and 0.075% tetrazolium solutions for 60, 90 and 120 minutes and maintained in an oven at 35 and 40 °C. Subsequently, the seeds were evaluated for viability, based on tissue color (bright red, pink or dark Carmine red). Simultaneously, a germination test was conducted, and its results were used as a reference for the tetrazolium test. The experiment was carried out in a completely randomized design, in a 3 x 3 + 1 factorial scheme (three concentrations of tetrazolium salt x three immersion periods, plus the control) in four replicates of 50 seeds for each treatment. The tetrazolium test to estimate okra seed viability should be conducted in 0.075% tetrazolium solution, for 90 minutes, at 35 °C.

The objective of this work was to evaluate the agronomic performance of erect and semi-erect cowpea elite lines grown in the States of Minas Gerais and Mato Grosso, Brazil. The experiments were conducted in Jaíba, Janaúba, Sete Lagoas (Minas Gerais), Primavera do Leste and Nova Ubiratã (Mato Grosso), with 20 genotypes of cowpea, arranged in a randomized block design with four replications. The data were subjected to individual and joint analyses of variance and means grouped by the Scott-Knott test (p<0.05). The plant growth habit, lodging, cultivation value, pod length and bean yield were evaluated. The lines MNC04-768F-21 and MNC04F-795F-168 had the highest bean yield in the experiments conducted in Mato Grosso, and their growth habit, lodging and cultivation value were similar to those of the cultivar BRS-Guariba. The lines MNC04-769F-30, MNC05-795F-154 and MNC04-769F-49 and the cultivar BRS-Tumucumaque had the highest bean yield in the experiments conducted in Minas Gerais. However, the cultivars BRS-Guariba, BRS-Tumucumaque, BRS-Novaera, BRS-Itaim and BRS-Cauamé and the lines MNC04-792F-143 and MNC04-792F-144 showed satisfactory bean yield and good growth habit, plant lodging and cultivation value in the State of Minas Gerais, especially in the northern region of the state (Jaíba and Janaúba), representing good alternatives for the genotypes recommended for this region.

Intercropping is a cropping system that involves cultivating two or more crops in the same area with different cycles and vegetative architectures. One of the ways to increase productivity and efficiency in this cropping system is through green manuring and by properly considering crop combinations, cultivation timing, and planting configuration of the component crops. Thus, the aim of this study was to evaluate the productive performance of carrot and lettuce intercropping systems under different amounts of rooster tree biomass incorporated into the soil and under different spatial arrangements. The study was conducted at the experimental farm Rafael Fernandes, RN, Brazil, in the period from September 2012 to January 2013, in an experimental design of randomized complete blocks in a 4 x 3 factorial scheme, with twelve treatments and 3 replicates. The treatments consisted of the combination of four rooster tree biomass amounts incorporated into the soil (10, 25, 40 and 55 t ha-1 on a dry basis) with three predetermined spatial arrangements between the component crops (2:2, 3:3 and 4:4), which correspond to the rows of carrot alternating with the rows of lettuce. The agronomic performance of the carrot and lettuce intercropping system was optimized in the amount of approximately 46.36 t ha-1 of rooster tree incorporated into the soil. There was no influence of the spatial arrangements on the agronomic efficiency of the carrot and lettuce intercropping. The use of rooster tree as green manure is agronomically viable in intercropped systems of carrot and lettuce.

ABSTRACT The interaction Fusarium oxysporum f. sp. cubense (Foc) x nematode and the genetic variability of Foc are the main problems with potential to affect the use of resistant varieties in the management of the Panama disease. The objective of this work was to evaluate the interaction between Foc and Meloidogyne javanica on the banana of the Prata Anã, Grande Naine and BRS Princesa cultivars. The experiment was conducted in a randomized block design, with six treatments and ten replications, which differed in the inoculation time of each pathogen. Simultaneous inoculation with Foc and M. javanica, inoculation with Foc one week before inoculation with M. javanica, and inoculation with M. javanica one week before inoculation with Foc. In addition to the controls, Foc isolated, M. javanica isolated, and cultivars without the pathogens. The seedlings were transplanted in 3-liter pots with sterile soil infested with 40 grams of Foc inoculum at the concentration of 1x106CFU/gram and 1000 eggs and J2 of M. javanica. At the end of 56 days, the treatments with 'Grande Naine' presented the highest reproduction factors of M. javanica. In the 'Prata Anã' no interaction was observed between Foc x M. javanica. The treatments with simultaneous inoculation of the two pathogens, and with Foc a week before, in the 'BRS Princesa', presented the highest external disease indices (DI), which promoted the highest AUDPC. The highest internal DI were observed in the treatments Foc+Mj+Grande Naine, Foc+Mj+BRS Princesa, Mj+Grande Naine+Foc and Foc+BRS Princesa+Mj. The simultaneous presence of M. javanica and Foc increases the severity of the Panama disease in Grande Naine and BRS Princesa cultivars. | RESUMO A interação Fusarium oxysporum f. sp. cubense (Foc) x nematoide e a variabilidade genética de Foc são os principais problemas com potencial de afetar o uso de variedades resistentes no manejo do mal-do-Panamá. O objetivo do trabalho foi avaliar a interação entre Foc e Meloidogyne javanica sobre as cultivares de bananeiras, Prata Anã, Grande Naine e BRS Princesa. O experimento foi conduzido em DBC, com seis tratamentos e dez repetições, os quais se diferenciaram no tempo de inoculação de cada patógeno. Inoculação simultânea de Foc e M. javanica, inoculação de Foc uma semana antes de M. javanica e inoculação de M. javanica uma semana antes de Foc. Além dos controles, Foc isolado, M. javanica isolado e cultivares sem patógenos. As mudas foram transplantadas em vasos de três litros com solo estéril infestado com 40 gramas de inóculo de Foc na concentração de 1x106UFC/grama e 1000 ovos e J2 de M. javanica. Ao final de 56 dias, os tratamentos em 'Grande Naine' apresentaram os maiores fatores de reprodução de M. javanica. Em 'Prata Anã' não foi observado interação entre Foc x M. javanica. Os tratamentos com inoculação simultânea dos dois patógenos e com Foc uma semana antes, em 'BRS Princesa', apresentaram os maiores índices de doença (ID) externos, o que proporcionou as maiores AACPD. Os maiores ID internos foram observados nos tratamentos, Foc+Mj+Grande Naine, Foc+Mj+BRS Princesa, Mj+Grande Naine+Foc e Foc+BRS Princesa+Mj. A presença conjunta de M. javanica e Foc aumenta a severidade do mal-do-Panamá nas cultivares Grande Naine e BRS Princesa.