RESUMO: O uso de bactérias fixadoras de nitrogênio do gênero Azospirillum brasilense tem sido recomendado para gramíneas como milho, trigo e arroz, porém seus benefícios necessitam ser melhor estudados. O objetivo deste trabalho foi avaliar o efeito da bactéria fixadora de nitrogênio A. brasilense na produtividade de arroz irrigado por inundação. O experimento foi conduzido em viveiro agrícola revestido com sombrite, utilizando um Plintossolo Háplico. No primeiro conjunto de experimentos utilizaram-se as cultivares de arroz irrigado por inundação INIA Olimar, Puitá Inta-CL, Br Irga 409 e Irga 424, constituindo 4 experimentos em delineamento experimental inteiramente casualizado, em esquema fatorial 5 x 4, com quatro repetições. Os tratamentos foram constituídos por 5 doses de nitrogênio (0, 40, 80, 120 e 160 kg ha-1 de N), e 4 doses de inoculante líquido composto por bactérias A. brasilense cepas Ab-V5 e Ab-V6 (0, 1, 2 e 4 vezes a recomendação). No segundo conjunto de experimentos, com as cultivares Puitá Inta-CL e Br Irga 409, utilizou-se o esquema fatorial 4 x 2. Quatro doses de A. brasilense cepas Ab-V5 e Ab-V6 (0, 1, 2 e 4 vezes a recomendação de 100 mL ha-1), sem e com tratamento das sementes do arroz com inseticidas e fungicidas. Foram avaliados a massa da matéria seca da parte aérea, número de panículas e produtividade de grãos de arroz/vaso. A inoculação das sementes de arroz irrigado por inundação, cultivares INIA Olimar, Puitá Inta-CL, Br Irga 409 e Irga 424, com A. brasilense cepas Ab-V5 e Ab-V6 não aumenta a produtividade de grãos de arroz. | ABSTRACT: This study aimed at evaluating the effect of Azospirillum brasilense, a nitrogen-fixing bacterium, on flooded irrigated rice yield. Evaluations were carried out in a shaded nursery, with seedlings grown on an Alfisol. Were performed two sets of experiments. In the first, were carried out four experiments using the flooded rice cultivars INIA Olimar, Puitá Inta-CL, Br Irga 409 and Irga 424; these trials were set up as completely randomized design in a 5x4 factorial scheme, with four replications. Treatments consisted of five nitrogen rates (0, 40, 80, 120 and 160 kg ha-1) and four levels of liquid inoculant Ab-V5 and Ab-V6 - A. brasilense (0, 1, 2 and 4 times the manufacturer's recommendation) without seed treatment. In second set, were performed two experiments using the cultivars Puitá Inta-CL and Br Irga 409, arranged in the same design, but using a 4x2 factorial. In this set, treatments were composed of four levels of Ab-V5 and Ab-V6 - A. brasilense liquid inoculant (0, 1, 2 and 4 times the recommendation of 100 mL ha-1), using rice seeds with and without insecticide and fungicide treatment. Shoot dry matter, number of panicles, and rice grain yield per pot were the assessed variables. The results showed that rice seed inoculation with A. brasilense had no effects on rice grain yield of the cultivars INIA Olimar, Puitá Inta-CL, Br Irga 409 and Irga 424.

The experiment was conducted in a greenhouse located at the Federal University of Mato Grosso, Cuiaba - MT, from March to May 2012. The objective was to assess the effects of different rates of nickel application with and without liming on maize growth and micronutrient levels. The study was a randomized block design in a 2 x 5 factorial arrangement with four replicates, for a total of 40 plots, including with and without liming and five rates of nickel application, on a clayey Red Yellow Latosol (Oxisol, USDA classification and Ferralsol, FAO classification). Both lime and nickel applications influenced plant growth, reducing plant development with increased nickel application without liming. It was also observed that both lime and nickel applications altered micronutrient levels in the maize plants, independent of which part of the plant was evaluated. Nickel played an antagonistic role with manganese and zinc and a synergistic role with copper and iron.

Water availability in the Brazilian semiarid is restricted and often the only water source available has high salt concentrations. Hydroponics allows using these waters for production of various crops, including vegetables, however, the water salinity can cause nutritional disorders. Thus, two experiments were conducted in a greenhouse at the Department of Agricultural Engineering of the Federal Rural University of Pernambuco, to evaluate the effects of salinity on the mineral nutrition of crisphead lettuce, cultivar Taina, in a hydroponic system (Nutrient Film Technique), using brackish water in the nutrient solution, which was prepared by adding NaCl to the local water (0.2 dS m-1). A randomized blocks experimental design was used in both experiments. The treatments consisted of water of different salinity levels (0.2, 1.2, 2.2, 3.2, 4.2 and 5.2 dS m-1) with four replications, totaling 24 plots for each experiment. The water added to compensate for the water-depth loss due to evapotranspiration (WCET) was the brackish water of each treatment in Experiment I and the local water without modifications in Experiment II. The increase in the salinity of the water used for the nutrient solution preparation reduced the foliar phosphorus and potassium contents and increased the chloride and sodium contents, regardless of the WCET. Foliar nitrogen, calcium, magnesium and sulfur contents were not affected by increasing the water salinity used for the nutrient solution preparation.

In recent years, the emergence of resistant pathogens has complicated the treatment of bacterial infections in livestock production as well as in the medical field, due to the development of resistance mechanisms by microorganisms. The objective of this study was to delineate the antimicrobial resistance profile of Salmonella spp. strains isolated from bivalve mollusks (oysters and mussels) and from estuarine environment water of two regions of Bahia, Brazil. Twenty-seven strains, 12 isolated from bivalve mollusks and 15 from estuarine water, were tested. Eight antimicrobial agents (phenicol, beta-lactams, tetracyclines, quinolones and fluoroquinolones classes) were used for a susceptibility test, Minimum Inhibitory Concentration (MIC) and extended-spectrum beta-lactamases (ESBLs) production. Isolates showed high susceptibility to the classes of antimicrobial agents tested, with resistance only to nalidixic acid (27%), ampicillin (25%) and tetracycline (25%). Bacterial resistance was of chromosomal origin and the multidrug resistance index (MAR) among isolates of shellfish (mussels in natura) was 0.25. The MIC was found to be 100 μg/mL, 500 μg/mL and 350 μg/mL to nalidixic acid, ampicillin and tetracycline, respectively. None of the isolates presented ESBLs production. The presence of multidrug-resistant and high MIC Salmonella spp. is being conveyed in extraction areas of bivalve mollusks in the State of Bahia, Brazil.

Seven biochemical descriptors were used to estimate the genotypic variability of peanut in response to moderate water stress. Six genotypes, constituted by four lines and two cultivars, were grown in pots, each containing two plants. At 15 days after emergence (DAE), the treatment differentiation was carried out: Control-plants maintained with daily watering, and Stress-plants submitted to water stress by complete suspension of watering for 15 days. The experimental design was completely randomized with factorial scheme 6 x 2 (genotype x water treatments), with five replications. The biochemical variables evaluated were: catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GPX), free proline, total carbohydrates, soluble proteins, and amino acids. Results obtained by biochemical analysis and estimation of genotypic variability indicated that proline is the most appropriate descriptor for selecting genotypes tolerant to water stress, which led to identification of L81V and L108V as promising lines for drought tolerance breeding program.

The northern region of Piauí state is an important center of irrigated organic fruit farming, wherein sandy soils with low organic matter content are predominant, besides climate with high temperatures, long periods of water deficit and constant wind. Given this situation, it becomes necessary to investigate alternative management options able to meet the challenges of agricultural productivity. In this context, green manuring becomes an important practice, mainly when using fast - growing species and with great potential for biomass production. Thus, a study was carried out at Embrapa Meio - Norte to measure Crotalaria juncea biomass yield after thinning and at various sowing densities. The evaluated parameters were plant height, stem diameter, number of branches per plant, fresh and dry weight of shoots and roots. The results showed that sowing density had no influence on plant height; however, unthinned plants reached greater heights, which might have been due to reduced competition for light. Thinning had no effect on diameters of plant stem base; on the other hand, lower planting densities promoted larger diameters also due to less competition. Likewise, the number of branches per plant was not affected by thinning, being greater at lower planting densities on account of less competition. Thinned plants achieved higher averages of fresh shoot weight. Nevertheless, shoot dry biomass was greater at higher planting densities. Furthermore, both fresh and dry weight of roots were not influenced by any of the adopted treatments.

The aim of this study was to evaluate the performance of progenies from Citrullus lanatus var. lanatus (cultivated watermelons) when crossed with progenies from C. lanatus var. citroides (fodder watermelon with a historic of resistance to the nematode Meloidogyne enterolobii). The parents and their F1s were evaluated for resistance to this nematode. In the initial stages of eleven treatments, watermelon seedlings plantlets were transplanted to plastic bags of six kilograms once the first leaves developed. Ten inoculated plants with 5,200 eggs in the soil near the stem of the plant and four non-inoculated ones were used in each treatment, in a complete block design. Sixty-two days after sowing, the following characteristics were evaluated: the length of the aerial part of the plant (LAP, in m), fresh mass of the aerial part (FMAP, in g), root fresh mass (RFM, in g), egg number (EN) and reproduction factor (RF). A comparison between the averages of inoculated and non-inoculated plants was performed using Scott-Knott test at 5% and the diallelic analysis was performed using the GENES program. The morphological characteristics did not allow for the identification of the parent plants or the F1s with respect to nematode resistance, but the variables EN and RF were useful for such identification. The analyses of the general and specific combining abilities indicate highly significant effects with respect to this resistance, showing additive gene effects as well as dominance and epistatic gene effects, allowing for identification of parents and F1s that can be used in watermelon breeding programs to improve resistance to the M. enterolobii.

The aim of the study was to evaluate the morphological and productive characteristics of five genotypes of Desmanthus spp. submitted to two cutting intensities (20 and 40 cm), in the semi-arid region of Pernambuco. Of the genotypes studied, three were derived from ecotypes collected in municipalities of Pernambuco (7G, 31D and 50J) and two were from the state of Sergipe, coming from Australia (10AU and 13AU). Four cuttings were made with a frequency of 60 days. The treatments were randomized in blocks, with split plots and three replications. The variables studied were plant height, stem diameter, canopy diameter, the number of leaves per branch, LAI, leaf yield, stem yield, pod yield and forage yield, and leaf stem-1 ratio and pod stem-1 ratio. Genotypes 7G and 31D had higher forage yield and a larger number of leaves per branch. Forage yield was higher in the intensity of 20 cm. The cuttings reduced the total yield, stem yield, pods yield and forage yield, of the different genotypes studied. For leaf stem-1 ratio, the genotype 13AU was higher than genotypes 7G and 50J in the cutting intensity of 40 cm. Genotype 10AU had the highest pod stem-1 ratios in the three cuttings. The differences shown between genotypes of Desmanthus spp., especially 7G and 31D, in the intensity of 20 cm, indicate the possibility of selecting promising materials for cultivation in the semi-arid region of Pernambuco, with desirable morphological and productive characteristics to the forage plants.

The cultivation of Litopenaeus vannamei has increased in inland areas by using low salinity waters. Cultivation techniques in coastal waters are well developed, however, studies for low salinity conditions are needed, especially regarding the ideal ionic composition of water and its effects on the zootechnical indices. Brazil has adopted this strategy and the semiarid region showed great potential due to its favorable climate and water conditions. Thus, experimental cultivations were conducted in Serra Talhada, State of Pernambuco, in order to evaluate the influence of two feeding strategies (with and without feeding) and three cultivation densities (5, 10 and 15 shrimps m-2), on the production variables of this species. Three ponds of 280 m2 were built, in which six nursery nets of 20 m2 were installed. The cultivation was performed with juvenile shrimps of 8.53 g and lasted 50 days. The animals were fed twice a day with commercial feed containing 25% of crude protein. The water quality (salinity ~1.0 g L-1, temperature ~28.09°C, pH ~8.48) and the shrimp growth were monitored with weekly biometry during the cultivation. A significant difference was found only in the treatment without feeding with 15 shrimps m-2, the average final weight of all treatments ranged from 13.16±1.33 g to 15.50±1.66 g. The survival rates were high in the treatments with feeding and its variation between treatments was 60.56±6.76% and 83.00±4.00%.

The Mangalarga Marchador breed accounts for the largest horse herd in Brazil and has been experiencing national and international expansion. Nevertheless, there is little scientific production on the evolution of this breed and there are no studies on the morphometric transformations that took place during its formation. In this context, the present study aimed to assess the evolution in morphometric measures of the Mangalarga Marchador breed between 1970 and 2010. The research used information archived at the Brazilian Association of Mangalarga Marchador Breeders from 23,148 males and 92,980 females in a completely randomized experimental design. The six treatments consisted of the animals measured between 1970 and 1979, 1980 and 1989, 1990 and 1994, 1995 and 1999, 2000 and 2004, and 2005 and 2010, respectively. The measures assessed were: height at withers and croup, length of the head, neck, back, croup, shoulder, and body, width of the head and croup, thoracic circumference, and cannon circumference Over the period analyzed, males and females experienced a reduction in thoracic circumference, in the lengths of the head, back, croup, and shoulder, and in the widths of the head and croup. In the males, neck length and cannon circumference also decreased, while body length increased in females. Over time, breed selection led to smaller head, higher stature, and shorter trunk length. However, important measures for the breed’s functionality, such as shoulder length and thoracic circumference, decreased.