Goat and sheep production in the semi-arid northeast of Brazil has shown great economic potential. However, health problems can compromise the productivity of these animals. Given the scarcity of studies about the occurrence of these diseases, the aim of the present study was to analyze the serological diagnosis of anti-Brucella and anti-lentivirus antibodies among small ruminants in municipalities located in the Brazilian states of Bahia and Pernambuco. The samples were collected from local slaughterhouses and dairy farms. In total, 997 serum samples from animals in slaughterhouses and dairy herds were collected. In order to diagnose the caprine arthritis encephalitis virus (CAEV), the samples underwent agarose gel immunodiffusion (AGID) testing. The buffered acidified antigen test (goats) and agarose gel immunodiffusion test (sheep) were used to detect anti-Brucella abortus and B. ovis antibodies following the methodology recommended by the Institute of Technology of Paraná (TECPAR). With anti-CAEV antibodies, seropositivity rates of 4.1% and 2.2% were recorded for animals from the slaughterhouses and dairy farms, respectively. None of the animals (goats or sheep) were positive for anti-B. abortus antibodies. With B. ovis, a seropositivity rate of 6.5% (n = 13) was recorded among the 199 sheep serum samples. Results of the present study confirmed the presence of the CAE virus in the meat and dairy herds studied, although the prevalence was low. Natural infection by B. abortus did not occur in the goat and sheep herds assessed. Seropositivity for B. ovis was confirmed, although prevalence was low. Direct tests are required to diagnose ovine brucellosis.

The present study aimed to investigate the temperature at different depths of the stack silo and strata front profile before and after the removal of silage. Also, the pH values in the upper and lower profile and the silage density of the fresh materials (FM) and dry matter (DM) in pearl millet silages with particle sizes of 5 or 20 mm, with or without inoculant, were assessed, using corn silage as controls. There was an interaction (P<0.05) silage × stratum profile and silage × depth profile for the temperatures before the removal of silage, and the pearl millet silages had lower temperatures in the upper stratum compared to corn. The temperature gradient between the silage and environment pearl millet and corn silos were smaller in the lower stratum profile. The temperature gradient after the removal of the silage was less than 50 cm deep at all the pearl millet silos. There were no differences in the densities of the FM and DM of the studied silages. The pH values of the silages before their removal were higher in the upper stratum and lower stratum in the bottom of all the pearl millet silages, in contrast with corn silage. The pearl millet silos had lower pH values in the lower stratum of the silo. Silages with 5 mm particle size provide lower temperatures in the middle portion of the panel before the removal of the silage. The use of bacterial inoculant in this study did not change the characteristics evaluated.

The etiologic agent of the Asian soybean rust is the Phakopsora pachyrhizi, which causes a reduction in the photosynthetic leaf area and, consequently, in the crop yield. Chemical control is one of the main measures for its management. The objective of this work was to evaluate the efficacy and selectivity of the fluxapyroxad fungicide on controlling the Asian soybean rust, under the edaphoclimatic conditions of the Cerrado biome. The experiment was conducted in an area under no-tillage system, in the Agricultural Research Center, Rio Verde, Goias, Brazil, during the 2012/2013 crop season, using the cultivar NA7337. A randomized block experimental design was used, with twelve treatments and four replications. The treatments consisted of applications of fluxapyroxad (FX), pyraclostrobin (PT), epoxiconazole (EX) and metconazole (MZ). The average severity of the disease in the plants reached 37% in the Control. All treatments with fungicides differed from the Control. Treatments 9, 10, 11 and 12 provided the greatest rates of soybean rust control. The treatments 10, 11 and 12 had the highest thousand grain weights, and the yields of the treatments 2, 3 and 11, despite higher than the Control, were lower than the treatments 4, 5, 6, 7, 8, 9, 10 and 12, which had statistically equal yields. The increasing in yield, compared to the Control, ranged from 10.05% (pyraclostrobin, epoxiconazole + pyraclostrobin + mineral oil) to 30.55% (pyraclostrobin, pyraclostrobin + fluxapyroxad + mineral oil and pyraclostrobin + metconazole + mineral oil). The highest rates of soybean rust control were presented by fungicides containing fluxapyroxad.

The experiment was conducted in a greenhouse located at the Federal University of Mato Grosso, Cuiaba - MT, from March to May 2012. The objective was to assess the effects of different rates of nickel application with and without liming on maize growth and micronutrient levels. The study was a randomized block design in a 2 x 5 factorial arrangement with four replicates, for a total of 40 plots, including with and without liming and five rates of nickel application, on a clayey Red Yellow Latosol (Oxisol, USDA classification and Ferralsol, FAO classification). Both lime and nickel applications influenced plant growth, reducing plant development with increased nickel application without liming. It was also observed that both lime and nickel applications altered micronutrient levels in the maize plants, independent of which part of the plant was evaluated. Nickel played an antagonistic role with manganese and zinc and a synergistic role with copper and iron.

The evaluation of land use and management by the measurement of soil organic matter and its fractions has gained attention since it helps in the understanding of the dynamics of their contribution to soil productivity, especially in tropical environments. This study was conducted in the municipality of Colorado do Oeste, state of Rondônia, Brazil and its aim was to determinethe quantity of organic carbon and total nitrogen in the light and heavy fractions of organic matter in the surface layers of a typic hapludalf under different land use systems: Native Forest: open evergreen forest, reference environment; Agroforestry System 1: teak (Tectona grandis LF) and kudzu (Pueraria montana); Agroforestry System 2: coffee (Coffea canephora), marandu palisade grass (Brachiaria brizantha cv. Marandu), “pinho cuiabano” (Parkia multijuga), teak and kudzu.; Agroforestry System 3: teak and cocoa (Theobroma cacao); Silvopasture System: teak, cocoa and marandu palisade grass; and Extensive Grazing System: marandu palisade grass. The experimental design was a randomized block in split-split plots (use systems versus soil layers of 0-0.05 and 0.05-0.10 m) with three replications. The results showed that relative to Native Forest, the Agroforestry System 2 had equal- and greater amounts of organic carbon and total nitrogen respectively (light and heavy fractions) in the soil organic matter, with the light fraction being responsible for storage of approximately 45% and 70% of the organic carbon and total nitrogen, respectively. Therefore, the light densimetric fraction proved to be useful in the early identification of the general decline of the soil organic matter in the land use systems evaluated.

In recent years, the emergence of resistant pathogens has complicated the treatment of bacterial infections in livestock production as well as in the medical field, due to the development of resistance mechanisms by microorganisms. The objective of this study was to delineate the antimicrobial resistance profile of Salmonella spp. strains isolated from bivalve mollusks (oysters and mussels) and from estuarine environment water of two regions of Bahia, Brazil. Twenty-seven strains, 12 isolated from bivalve mollusks and 15 from estuarine water, were tested. Eight antimicrobial agents (phenicol, beta-lactams, tetracyclines, quinolones and fluoroquinolones classes) were used for a susceptibility test, Minimum Inhibitory Concentration (MIC) and extended-spectrum beta-lactamases (ESBLs) production. Isolates showed high susceptibility to the classes of antimicrobial agents tested, with resistance only to nalidixic acid (27%), ampicillin (25%) and tetracycline (25%). Bacterial resistance was of chromosomal origin and the multidrug resistance index (MAR) among isolates of shellfish (mussels in natura) was 0.25. The MIC was found to be 100 μg/mL, 500 μg/mL and 350 μg/mL to nalidixic acid, ampicillin and tetracycline, respectively. None of the isolates presented ESBLs production. The presence of multidrug-resistant and high MIC Salmonella spp. is being conveyed in extraction areas of bivalve mollusks in the State of Bahia, Brazil.

The feeding frequencies on pacamã Lophiosilurus alexandri larviculture were evaluated. In the first phase, 10 days of active feeding, larvae with nine days post hatching (13.5 ± 0.7 mm) were stocked at density of 10 larvae L-1 in 5-L tanks each. The experimental treatments were: F2T (feeding 8h00 and 17h00); F2M (feeding 8h00 and 12h30); F3 (feeding 8h00, 12h30 and 17h00); and F4 (feeding 8h00, 11h00, 14h00 and 17h00). During this phase Artemia nauplii were used as food. In the second phase, during 15 days, juveniles were restocked in 20-L tanks at density of 2.2 juveniles L-1, and kept the same feeding frequencies of the first phase. Commercial dry diet containing 40% crude protein was used as food. No effect of feeding frequency on growth and survival, during both phases, was observed. However, survival decreases from the first to the second phase, showing the importance of the type of food. During the L. alexandri larviculture should be recommended twice a day feeding frequency.

Jatropha has emerged as a source of power generation in the semiarid region of Brazil. The objective of this study was to evaluate, using geostatistics, the spatial variability of stem diameter, plant height and seed weight of jatropha (Jatropha curcas L.) grown under rain fed conditions in Serra Talhada, Pernambuco state, Brazil. At 545, 905 and 1265 days after sowing (DAS), the stem diameter and plant height of 340 plants were measured with a digital caliper and a tape measure. At 545 DAS, the fruits of 306 plants were collected, the seeds extracted and their weights measured with a precision balance. For each date analyzed, the spatial dependence of the plant characteristics was evaluated from the adjustment of the experimental semivariogram. The spherical model was adjusted for modeling the spatial distribution of cultural parameters. The range of plant height reduced by an average of 37% from 545 DAS when compared to 905 and 1265 DAS, while for the stem diameter this parameter remained practically constant. Geostatistics can be employed to assist in future sampling and to identify areas with different patterns that can occur in the jatropha planting.

Os sistemas de irrigação por gotejamento se destacam para a aplicação de águas residuárias, devido à minimização das perdas de água, dos riscos ambientais e de saúde pública, porém a obstrução dos emissores representa um problema potencial dessa tecnologia. Objetivou - se com este trabalho avaliar o coeficiente de variação de vazão (FCV) e o coeficiente de uniformidade estatístico (Us) de unidades gotejadoras abastecidas com percolado de aterro sanitário diluído em água de abastecimento. O experimento foi conduzido sob delineamento inteiramente casualizado no esquema de parcelas subdivididas, tendo nas parcelas os tipos de gotejadores (G1 não autocompensante; e G2, G3 e G4 autocompensantes) e nas subparcelas os tempos de avaliação (0, 20, 40, 60, 80, 100, 120, 140 e 160 horas), com quatro repetições. Os valores de FCV, CSU e das características físico - químicas e biológicas do percolado de aterro sanitário diluído foram determinadas a cada 20 horas, até completar 160 horas de operação das unidades gotejadoras. A bioincrustação proporcionou maiores alterações nos valores de FCV e CSU da unidade gotejadora com gotejador G1 (não autocompensante) em relação às unidades dotadas dos gotejadores G2, G3 e G4 (autocompensantes). O gotejador G3 foi o mais adequado na aplicação do percolado de aterro sanitário diluído.

The objective of this work was to survey the entomofauna in an experimental vineyard ( Vitis spp.) in the semiarid of the State of Rio Grande do Norte (RN), Brazil. The survey was conducted from November 2012 to January 2013 at the Rafael Fernandes experimental farm of the Universidade Federal Rural do Semi - Árido (UFERSA), Mossoró, RN, in an area of 2,500 m 2 , cultivated with the Italia , Niagara Rosada and Isabel grapevine varieties. The insect collection was performed weekly during the grapevine flowering and fruiting periods, through a passive method, using four Pitfall traps, and an active method, collecting insects from the tree canopies (branches, leaves, flowers and fruits) using entomological net, tweezers and brushes. In the passive collection, 1,598 insects from five orders and 25 families were collected, especially from the Formicidae (83%), Dolichopodidae (5.9%) and Cicadellidae (1.9%) families, which were the most abundant. In the active collection, 288 insects from five orders and 14 families were collected, especially from the Vespidae (160 specimens) and Apidae (78 specimens) families, which were the most abundant, representing 82.7% of the total collected insects. The greater diversity (13 families) and greater number of insects collected (186 specimens) were observed in the grapevine variety Isabel.