An integrated two-phase AD with acidogenic off-gas diversion from a leach bed reactor to an upflow anaerobic sludge blanket was developed for improving methane production. However, this system had its own technical limitation such as mass transfer efficiency for solid-state treatment. In order to optimize the mass transfer in this two phase AD system, leachate recirculation with various water replacement rates regulating the total solids contents (TS) at 12.5%, 15%, and 17.5% was aim to investigate its effect on methane generation. The solubilization of food waste was increased with decreasing TS content, while the enzymatic hydrolysis showed the opposite trend. A TS contents of 15% presented the best acidogenic performance with the highest hydrogen yield of 30.3 L H₂/kg VSₐddₑd, which subsequently resulted in the highest methane production. The present study provides an easy approach to enhance food waste degradation in acidogenic phase and energy conversion in methanogenic phase simultaneously.

This study investigated the enhancement effect of N₂- and Air-nanobubble water (NBW) supplementation on two-stage anaerobic digestion (AD) of food waste (FW) for separate production of hydrogen and methane. In the first stage for hydrogen production, the highest cumulative H₂ yield (27.31 ± 1.21 mL/g-VSₐddₑd) was obtained from FW + Air-NBW, increasing by 38% compared to the control (FW + deionized water (DW)). In the second stage for methane production, the cumulative CH₄ yield followed a descending order of FW + Air-NBW (373.63 ± 3.58 mL/g-VSₐddₑd) > FW + N₂-NBW (347.63 ± 7.05 mL/g-VSₐddₑd) > FW + DW (300.93 ± 3.24 mL/g-VSₐddₑd, control), increasing by 24% in FW + Air-NBW and 16% in FW + N₂-NBW compared to the control, respectively. Further investigations indicate that different gas–NBW may positively impact the different stages of AD process. Addition of N₂-NBW only enhanced the hydrolysis/acidification of FW with no significant effect on methanogenesis. By comparison, addition of Air-NBW promoted both hydrolysis/acidification stage and methanogenesis stage, reflecting by the enhanced activities of four extracellular hydrolases at the end of hydrolysis/acidification and coenzyme F₄₂₀ at the end of methanogenesis, respectively. Results from this work suggest the potential application of Air-NBW in the two-stage AD for efficient renewable energy recovery from FW.