The agricultural sector serves as the cornerstone of the Kenyan economy, wielding significant economic influence by directly contributing 33% of the Gross Domestic Product (GDP), and another 27% of the GDP indirectly through linkages with other sectors (FAO, 2025). The sector accounts for 65% of total export earnings and sustains informal livelihoods of more than 80% of the Kenyan population in meeting employment, income and food security needs and contributes to improving nutrition through production of safe, diverse and nutrient dense foods. The sector also employs over 40% of the total population and more than 70% of the rural population (Central Bank of Kenya, 2023; World Bank Group 2021). The sector is also the main driver of the non-agricultural economy including manufacturing, providing inputs and markets for non-agricultural operations such as building or construction, transportation, tourism, education and other social services. This underscores the pivotal role of the agricultural sector in driving Kenya’s economy and providing livelihoods for a substantial portion of the population. Climate change poses significant threat to the Kenyan economy due to its dependence on natural resources such as water for food and energy, and the country’s exposure to climate-sensitive sectors, especially agriculture. The rising frequency of extreme weather events is the main driver of climate change-related impact on the Kenyan economy. Kenya’s agricultural sector is heavily reliant on rain-fed smallholder subsistence farming, which exposes the sector to profound impacts of climate change. Increasing variability of rainfall patterns, increasing temperature interspersed with prolonged drought periods and flash floods directly affecting crop and livestock productivity. This impact is varied across the country with higher temperatures having a positive impact in highland areas but a negative effect in lowland areas, with this being pronounced in the ASAL regions. Smallholder farmers who make up majority of Kenyan farmers, are highly vulnerable to climatic and environmental hazards as their options for diversifying their resources and income sources are limited. Droughts negatively affect access to credit for agriculture, as the value and availability of farm assets to serve as collateral decline. Further, their vulnerability is worsened by lack of access to adequate water, low levels of technology and education and institutional mismanagement. These challenges exacerbate the vulnerability and dampen adaptive capacity of smallholder farmers to climate shocks, particularly in the food basket regions.

International audience | The increasing demand for food and especially proteins leads to the search for alternative protein sources. Meat co-products, which are available but little used in human food, provide a potential solution to this challenge. The present study aimed to evaluate the nutritional quality of two beef protein ingredients (greasy greaves recovered proteins (GGRP) and water recovered proteins (WRP)), both co-products of the fat rendering process. Their true ileal digestibility (TID), digestible indispensable amino acid score (DIAAS) and kinetics of plasma amino acids (AA) were measured in ten growing pigs, each fed the two co-products and a protein-free diet. Titanium dioxide was used as an indigestible marker. Digesta samples were collected for 9 h after meal ingestion, and blood samples were collected at ten time points during the same period. Total nitrogen (N) and AA contents were determined. Data were statistically analysed using linear mixed models. The TID of total N was not different between WRP and GGRP (81-84 %, P > 0•05). The first-limiting AA was Trp for both ingredients, with a DIAAS much higher for GGRP than for WRP (74 and 10 % for adults, respectively; P < 0•001). Postprandial plasma AA concentration peaked earlier for WRP (3 h) than for GGRP (5 h). Plasma concentrations of total and essential AA were higher (P < 0•001) with GGRP diet than WRP diet. Overall, GGRP has a nutritional quality suitable to meet the needs of adults for AA, while WRP needs to be supplemented with other protein sources to fulfil the dietary requirements.

The implementation of the Grain for Green Project has increased vegetation coverage and provided suitable habitats and food resources for soil fauna, thereby promoting the development of soil faunal communities. Studying seasonal variations in soil fauna communities in different vegetation areas can improve our understanding of the mechanisms that drive soil fauna recovery. We selected five typical artificially restored vegetation habitats, including <i>Populus simonii</i> (POS), <i>Pinus tabulaeformis</i> (PIT), <i>Caragana korshinskii</i> (CAK), <i>Stipa bungeana</i> (STB), and <i>Medicago sativa</i> (MES), and one farmland (<i>Zea mays</i>, FAL) habitat on the Loess Plateau. In this study, soil fauna communities and environmental factors were investigated during spring (May), summer (August), and autumn (November). Among the habitats, the STB habitat had the largest seasonal variation in soil faunal density (from 1173 ind·m⁻² in May to 10,743 ind·m⁻² in August), and the FAL habitat had the smallest (from 2827 ind·m⁻² in August to 5550 ind·m⁻² in November). Among the restored vegetation habitats, Acarina (44.89–88.56%) had the highest relative abundance of all taxa. The redundancy analysis (RDA) results showed that among the factors driving seasonal variation in soil animal communities, temperature (47.41%) was the most important, followed by precipitation (22.60%). In addition, the dominant groups, Acarina and Collembola, played an influential role in seasonal variations in soil faunal density. Temperature mainly determined the seasonal variations in soil faunal communities. Seasonal factors should be considered when conducting soil fauna research, as they contribute to biodiversity conservation and regional ecological management in the Loess Plateau.

The national climate action plans to cut greenhouse gas emissions, referred to as Nationally Determined Contributions (NDCs), currently include anthropogenic land-based carbon fluxes but typically exclude open ocean carbon sinks within Exclusive Economic Zones (EEZs). Here, we utilise a high-resolution global ocean biogeochemical model alongside available observations to illustrate why including EEZ carbon sinks in NDCs would be both conceptually and practically highly problematic. To demonstrate this, we focus on the case of the French EEZ, where we simulate an anthropogenic carbon sink of 0.21 GtCO2·yr–1 over the past decade, representing nearly 50% of France’s territorial greenhouse gas emissions over the same period. However, it should be noted that this anthropogenic carbon uptake results from the physical and biogeochemical properties of water masses, rather than from human management practices. Additionally, the EEZ oceanic carbon sink is already considered in estimates of remaining carbon budgets to meet the goals of the Paris Agreement. Therefore, integrating these fluxes into NDCs would lead to inconsistency between national objectives and global climate goals. We argue that to identify the additionality of management measures aimed at enhancing the anthropogenic carbon sink in EEZ waters, robust assessments of the baseline carbon sink and how it would have evolved without intervention will be needed. As we show, this is complicated by the spatially and temporally dynamic nature of ocean carbon fluxes. More fundamentally, there is no colocalization between air–sea fluxes and the subsequent sequestration of carbon in the ocean due to circulation and mixing. Consequently, the measurement, reporting, and verification requirements for national stocktakes would be highly challenging. Moreover, as fluxes predominantly occur in the high latitude oceans and sequestration in the subtropics, the national allocation of credits would involve contentious issues of equity and justice. | Les plans d’action nationaux pour le climat, connus sous le nom de contributions déterminées au niveau national (CDN), intègrent les flux de carbone anthropiques continentaux, mais excluent généralement le puit de carbone océanique des zones économiques exclusives (ZEE). Dans cette étude, nous utilisons un modèle biogéochimique océanique global à haute résolution ainsi que les observations disponibles pour illustrer les raisons pour lesquelles l’intégration du puits de carbone des ZEE dans les CDN serait conceptuellement et concrètement problématique. Pour le démontrer, nous nous penchons sur le cas de la ZEE française, pour laquelle nous simulons un puits de carbone anthropique de 0,21 GtCO2·yr–1 au cours des dix dernières années. Nous argumentons que pour identifier l’additionnalité de mesures d’atténuation, des évaluations solides du puits de carbone de référence sont nécessaires. Cette tâche est rendu complexe, comme nous le d’emontrons, en raison de la nature dynamique des flux de carbone océaniques.

This work presents an analysis of the relationship between strength parameters determined in the laboratory and the results of a cone penetration test with pore water pressure measurement (CPTU) of waste soils in the “White Seas” area in Cracow. Anthropogenic soil is an alkaline waste formed during the production of soda ash and deposited in the area of the former Solvay Sodium Plant factory in Cracow, Poland. Due to the large area of the land and numerous investment plans and completed buildings, there was a need to identify reliable functional relationships enabling the determination of the strength parameters of these soils based on the results of the CPTU. Statistical analysis showed that the best correlation with the test results was provided by two logarithmic functions in which the dependent variables were the effective friction angle and effective cohesion. The dependent variable for both cases was the corrected cone resistance q_t. The functional relationship combined data from labour-intensive, long-lasting and costly laboratory measurements with quick and less expensive measurements, i.e., in situ CPTUs. The obtained relationships enable the determination of the strength properties of the subsoil of these anthropogenic soils.

This study determined the physicochemical properties of raw milk according to the milking system used. The raw material milk was collected from 10 holdings with different forms of ownership, with different technical equipment and different milking systems. Raw material milk parameters were analyzed: fat, protein, lactose, non-fat dry substance by the rapid method with the Bentley 150 analyzer.The milk from the mechanized systems is of high physicochemical quality, recording values of the quality parameters without much variation almost throughout the year, regardless of the dominant breed and the milking equipment used.

Although pharmaceuticals are increasingly detected in abiotic matrices in the Arctic, the accumulation of drugs in the resident biota and trophic transfer have not been yet examined. This study investigated the behaviour of several pharmaceuticals in the rocky-bottom, macrobenthic food web in the coastal zone of Isfjorden (western Spitsbergen) using stable isotope analyses (SIA) coupled with liquid chromatography-mass spectrometry (LC-MS/MS). Across 16 macroalgal and invertebrate species the highest average concentration was measured for ciprofloxacin (CIP) (on average 60.3 ng g−1 dw) followed by paracetamol (PCT) (51.3 ng g−1 dw) and nicotine (NIC) (37.8 ng g−1 dw). The biomagnification potential was assessed for six target compounds of 13 analytes detected that were quantified with a frequency > 50 % in biological samples. The trophic magnification factor (TMF) ranged between 0.3 and 2.8, and was significant for NIC and CIP. TMF < 1.0 for NIC (0.3; confidence interval, CI 0.1–0.5) indicated that the compound does not accumulate with trophic position. The dilution of pharmaceutical residues in the food web may result from limited intake with dietary route, poor assimilation efficiency and high biotransformation rates in benthic invertebrates. TMF for CIP (2.8, CI 1.2–6.4) suggests trophic magnification, a phenomenon observed previously for several antibiotics in freshwater food webs. Trophic transfer therefore plays a role in controlling concentration of CIP in the Arctic benthic communities and should be considered in environmental risk assessment. Biomagnification potential of diclofenac (DIC; 0.9, CI 0.5–1.7), carbamazepine (CBZ; 0.4, CI 0.1–2.1), caffeine (CAF; 0.9, CI 0.5–1.9) and PCT (1.3, CI 0.7–2.7) was not evident due to large 95 % confidence of their TMFs. This study provides the first evidence of drug bioaccumulation in the Arctic food web and indicates that behaviour of pharmaceuticals varies among target compounds. | publishedVersion

Growth of meat microbiota usually results in spoilage of meat that can be perceived by consumers due to sensory changes. However, a high bacterial load does not necessarily result in sensory deviation of meat; nevertheless, this meat is considered unfit for human consumption. Therefore, the aims of this study were to investigate changes in the microbiota from fresh to spoiled meat and whether the proportions of certain bacteria can probably be used to indicate the hygiene status of meat. For this purpose, 12 fresh pork samples were divided into two groups, and simultaneously aerobically stored at 4°C and 22°C. At each time-temperature point (fresh meat, days 6, 13, and 20 at 4°C, and days 1, 2, 3, and 6 at 22°C), 12 meat subsamples were investigated. Sequences obtained from next-generation sequencing (NGS) were further analyzed down to species level. Plate counting of six bacterial groups and NGS results showed that Pseudomonas spp. and lactic acid bacteria (LAB) were found in a high proportion in all stored meat samples and can therefore be considered as important “spoilage indicator bacteria”. On the contrary, sequences belonging to Staphylococcus epidermidis were found in a relatively high proportion in almost all fresh meat samples but were less common in stored meat. In this context, they can be considered as “hygiene indicator bacteria” of meat. Based on these findings, the proportion of the “hygiene indicator bacteria” in relation to the “spoilage indicator bacteria” was calculated to determine a “hygiene index” of meat. This index has a moderate to strong correlation to bacterial loads obtained from culture (p < 0.05), specifically to Pseudomonas spp., LAB and total viable counts (TVCs). Knowledge of the proportions of hygiene and spoilage indicator bacteria obtained by NGS could help to determine the hygiene status even of (heat-) processed composite meat products for the first time, thus enhancing food quality assurance and consumer protection.

ABSTRACTWe analyzed the pan-genome and gene content modulation of the most diverse genome data set of the Mycobacterium tuberculosis complex (MTBC) gathered to date. The closed pan-genome of the MTBC was characterized by reduced accessory and strain-specific genomes, compatible with its clonal nature. However, significantly fewer gene families were shared between MTBC genomes as their phylogenetic distance increased. This effect was only observed in inter-species comparisons, not within-species, which suggests that species-specific ecological characteristics are associated with changes in gene content. Gene loss, resulting from genomic deletions and pseudogenization, was found to drive the variation in gene content. This gene erosion differed among MTBC species and lineages, even within M. tuberculosis, where L2 showed more gene loss than L4. We also show that phylogenetic proximity is not always a good proxy for gene content relatedness in the MTBC, as the gene repertoire of Mycobacterium africanum L6 deviated from its expected phylogenetic niche conservatism. Gene disruptions of virulence factors, represented by pseudogene annotations, are mostly not conserved, being poor predictors of MTBC ecotypes. Each MTBC ecotype carries its own accessory genome, likely influenced by distinct selective pressures such as host and geography. It is important to investigate how gene loss confer new adaptive traits to MTBC strains; the detected heterogeneous gene loss poses a significant challenge in elucidating genetic factors responsible for the diverse phenotypes observed in the MTBC. By detailing specific gene losses, our study serves as a resource for researchers studying the MTBC phenotypes and their immune evasion strategies.IMPORTANCEIn this study, we analyzed the gene content of different ecotypes of the Mycobacterium tuberculosis complex (MTBC), the pathogens of tuberculosis. We found that changes in their gene content are associated with their ecological features, such as host preference. Gene loss was identified as the primary driver of these changes, which can vary even among different strains of the same ecotype. Our study also revealed that the gene content relatedness of these bacteria does not always mirror their evolutionary relationships. In addition, some genes of virulence can be variably lost among strains of the same MTBC ecotype, likely helping them to evade the immune system. Overall, our study highlights the importance of understanding how gene loss can lead to new adaptations in these bacteria and how different selective pressures may influence their genetic makeup.

The goals of the Paris Agreement (PA) on collectively managing climate change can only be reached if all parties to the United Nations Framework Convention on Climate Change (UNFCCC) commit to actions supporting their Nationally Determined Contributions (NDCs). Developing-economy nations play a crucial role in reaching the PA targets, particularly in the Agriculture, Forest, and Other Land Uses (AFOLU) sector. However, developing country Parties also face several constraints in tracking and communicating progress towards their climate policy targets and implementation of their NDCs. The operationalization of Biennial Transparency Report (BTR) and Enhanced Transparency Framework (ETF) under the PA will bring stricter reporting timeframes and advanced transparency for all parties. With these requirements rapidly coming into force, addressing reporting gaps is now a pressing priority. The present study analyzes the NDCs, and Biennial Update Reports (BURs) submitted by developing country Parties under the UNFCCC. In an illustrative exercise, our in-depth analysis concentrates on reporting on the AFOLU sector and identifies issues impeding a comprehensive and comparable Global Stock Take (GST): (i) issues of consistency in reporting timeframes (ii) issues in transparency of reporting on mitigation sectors and on relevant progress indicators (iii) incomparability of methodological approaches proposed and used, and (iv) the implications of limited national capacity for transparent reporting. The UNFCCC and developed country Parties now have the opportunity of providing specialized support for developing country Parties. This could include tailored guidance to address gaps in both greenhouse-gas (GHG) emissions accounting, and reporting challenges, to ensure consistent, comprehensive, and transparent reporting to reinforce capacities moving forward following the next GST.