Seeds of Cistus ladanifer experience bursts of germination following fires. The effects of heat shock from 10 °C to 150 °C on seed germination were investigated by final germination plus the number of days required for germination to start and finish, and symmetry of cumulative germination. The occurrence of physical dormancy in C. ladanifer seeds was investigated by a variety of methods, including imbibition, scanning electron microscopy (SEM) and light microscopy, and use of dyes. The significance of responses of C. ladanifer seeds to fires was investigated essentially by abstracting existing literature and by using fire effects models and simulations. Parameters of germination were variously affected by heat treatments—positively in the range 80–100 °C, negatively above 130 °C. Non-dormancy was consistently found in about 30% of seeds but no evidence was obtained to support the existence of physical dormancy in the dormant fraction of C. ladanifer seeds. Two complementary processes seem to be in place in seeds response to fire. A direct fire-driven increase in germination of virtually all seeds in response to the appropriate heat load produced by fire or, in the absence of such heat loads, the germination of the non-dormant fraction provided that above-ground vegetation burns.

Seeds of Cistus ladanifer experience bursts of germination following fires. The effects of heat shock from 10 °C to 150 °C on seed germination were investigated by final germination plus the number of days required for germination to start and finish, and symmetry of cumulative germination. The occurrence of physical dormancy in C. ladanifer seeds was investigated by a variety of methods, including imbibition, scanning electron microscopy (SEM) and light microscopy, and use of dyes. The significance of responses of C. ladanifer seeds to fires was investigated essentially by abstracting existing literature and by using fire effects models and simulations. Parameters of germination were variously affected by heat treatments—positively in the range 80–100 °C, negatively above 130 °C. Non-dormancy was consistently found in about 30% of seeds but no evidence was obtained to support the existence of physical dormancy in the dormant fraction of C. ladanifer seeds. Two complementary processes seem to be in place in seeds response to fire. A direct fire-driven increase in germination of virtually all seeds in response to the appropriate heat load produced by fire or, in the absence of such heat loads, the germination of the non-dormant fraction provided that above-ground vegetation burns.

ABSTRACT The objective was to evaluate the physical characteristics and physiological behavior of A. oleracea seeds during imbibition and germination, under different temperatures and photoperiods, as well as to determine the duration of the germination test. For this, biometric parameters (length, width and thickness) were measured in 100 seeds and thousand-seed weight was determined. In addition, the external structures of seeds and seedlings were described and the imbibition curve was monitored. Subsequently, the germination test was conducted at temperatures of 20, 25, 30, 35, 20-30 and 25-35 ºC, under constant and alternating light and dark photoperiods (12 h), arranged in 6 x 3 factorial scheme, with four replicates of 50 seeds. Seeds were sown in transparent plastic boxes with blotting paper as substrate, germinated in Biochemical Oxygen Demand (B.O.D) incubator, and evaluated for six days. The following variables were analyzed: germination, germination speed index, seedling length and seedling dry mass. The imbibition pattern test was conducted at 25 ºC with 4 replicates of 100 seeds, evaluated until 50% root protrusion occurred. A. oleracea seeds exhibit uniform physical characteristics and the three-phase model as a water absorption pattern. Constant temperatures of 25 and 30 °C and alternating temperatures of 20-30 °C, regardless of the photoperiod, led to higher germination values. For A. oleracea, the germination test evaluations can be performed on the fourth and sixth day after sowing, as first and last counts, respectively.

ABSTRACT The objective was to evaluate the physical characteristics and physiological behavior of A. oleracea seeds during imbibition and germination, under different temperatures and photoperiods, as well as to determine the duration of the germination test. For this, biometric parameters (length, width and thickness) were measured in 100 seeds and thousand-seed weight was determined. In addition, the external structures of seeds and seedlings were described and the imbibition curve was monitored. Subsequently, the germination test was conducted at temperatures of 20, 25, 30, 35, 20-30 and 25-35 ºC, under constant and alternating light and dark photoperiods (12 h), arranged in 6 x 3 factorial scheme, with four replicates of 50 seeds. Seeds were sown in transparent plastic boxes with blotting paper as substrate, germinated in Biochemical Oxygen Demand (B.O.D) incubator, and evaluated for six days. The following variables were analyzed: germination, germination speed index, seedling length and seedling dry mass. The imbibition pattern test was conducted at 25 ºC with 4 replicates of 100 seeds, evaluated until 50% root protrusion occurred. A. oleracea seeds exhibit uniform physical characteristics and the three-phase model as a water absorption pattern. Constant temperatures of 25 and 30 °C and alternating temperatures of 20-30 °C, regardless of the photoperiod, led to higher germination values. For A. oleracea, the germination test evaluations can be performed on the fourth and sixth day after sowing, as first and last counts, respectively. | RESUMO Objetivou-se avaliar as características físicas e o comportamento fisiológico de sementes de A. oleracea durante a embebição e germinação, sob diferentes temperaturas e fotoperíodos, bem como determinar a duração para a execução do teste de germinação. Para isso, realizaram-se a biometria das sementes (comprimento, largura e espessura) de 100 sementes e peso de mil sementes. Assim como, as descrições das estruturas externas das sementes e plântulas e o monitoramento da curva de embebição. Em seguida, realizou-se o teste de germinação sob temperaturas de 20, 25, 30, 35, 20-30, e 25-35 ºC, submetidas aos fotoperíodos de luz e escuro constantes e alternado (12 h), arranjados em fatorial 6 x 3, com quatro repetições de 50 sementes. As sementes foram semeadas em caixas de plástico transparente, tendo como substrato o papel mata-borrão e colocadas para germinar em Biochemical Oxygen Demand (B.O.D), sendo avaliadas durante seis dias. As variáveis analisadas foram: germinação, índice de velocidade de germinação, comprimento e massa seca de plântulas. O padrão de embebição, foi realizado com 4 repetições de 100 sementes, a 25 ºC, e avaliado até o surgimento de 50% de protrusão radicular. As sementes de A. oleracea apresentam características físicas uniformes e o modelo trifásico como padrão de absorção de água. As temperaturas de 25 e 30 °C e alternada de 20-30 °C, independente do fotoperíodo, proporcionaram maiores valores de germinação. As avaliações do teste de germinação de A. oleracea podem ser realizadas no quarto e sexto dia após a semeadura, como primeira e última contagem, respectivamente.

This study evaluated the physical and physiological attributes of saved cowpea seeds (Vigna unguiculata L. Walp.) used in the Brazilian semi-arid area and compared them with certified seed varieties. The study tested 37 saved seed samples from the state of Rio Grande do Norte and two varieties of certified seeds (“BRS Guariba” and “BRS Pujante”) from the production field of Embrapa Products and Market, Petrolina, PE, Brazil. The seeds were tested for moisture, hectoliter weight, weight of 1,000 seeds, and physical purity to evaluate the physical quality. Germination, first germination count, field emergence, emergence rate index, shoot dry mass, and accelerated aging were tested for physiological quality. The saved cowpea seeds showed great differences, suggesting the influence of genetic variability and different growing environments. The use of saved cowpea seeds by small farmers in the semi-arid areas of Northeastern Brazil is impractical, because these seeds have inferior physical and physiological qualities compared to certified cowpea seeds.

RESUMO Objetivou-se avaliar a qualidade física e fisiológica de sementes salvas de feijão-caupi (Vigna unguiculata L. Walp.) utilizadas no semiárido brasileiro e compará-las com as variedades de sementes certificadas. Foram testadas 37 amostras de sementes salvas oriundas de diferentes municípios do Rio Grande do Norte e duas variedades de sementes certificadas (BRS Guariba e BRS Pujante), provenientes do campo de produção da Embrapa Produtos e Mercado, Petrolina, PE. Para avaliação da qualidade física, as sementes foram submetidas aos testes de grau de umidade, peso hectolítrico, peso de mil sementes e pureza física. Na avaliação da qualidade fisiológica testou-se a germinação, primeira contagem de germinação, emergência em campo, índice de velocidade de emergência, matéria seca de parte aérea e envelhecimento acelerado. As sementes salvas de feijão-caupi apresentaram grandes diferenças, sugerindo tanto a influência da variabilidade genética como dos diferentes ambientes de cultivo. A utilização de sementes salvas de feijão-caupi pelos pequenos produtores das áreas semiáridas do nordeste do Brasil se constitui em prática inviável por se tratar de sementes de qualidades física e fisiológica inferiores às sementes de origem certificadas. | ABSTRACT This study evaluated the physical and physiological attributes of saved cowpea seeds (Vigna unguiculata L. Walp.) used in the Brazilian semi-arid area and compared them with certified seed varieties. The study tested 37 saved seed samples from the state of Rio Grande do Norte and two varieties of certified seeds (“BRS Guariba” and “BRS Pujante”) from the production field of Embrapa Products and Market, Petrolina, PE, Brazil. The seeds were tested for moisture, hectoliter weight, weight of 1,000 seeds, and physical purity to evaluate the physical quality. Germination, first germination count, field emergence, emergence rate index, shoot dry mass, and accelerated aging were tested for physiological quality. The saved cowpea seeds showed great differences, suggesting the influence of genetic variability and different growing environments. The use of saved cowpea seeds by small farmers in the semi-arid areas of Northeastern Brazil is impractical, because these seeds have inferior physical and physiological qualities compared to certified cowpea seeds.