Properties of glutamate dehydrogenase in developing legume fruit, 2: the effects of divalent cations [Lupinus angustifolius]
1983
Marziah, Mahmood (Univeriti Pertanian Malaysia, Serdang, Selangor. Faculty of Science and Environmental Studies)
The activity of EDTA-inhibited glutamate dehydrogenase from a crude seed extract of Lupinus angustifolius could be partially or fully restored by the addition of divalent cations to the incubation mixture. Mn2+ and Ca2+ gave the highest stimulation of 67% and 66% of the control, respectively; followed by Zn2+ (59%), Co2+ (55%), Mg2+ (53%) and Cu2+ (49%). The Mn-deficient enzyme was more sensitive to manganese and more stimulated at a lower manganese concentration than the Mn-sufficient enzyme. However, Mn-deficient enzyme achieved a maximum activity of 91% at 1.4mM Mn2+ while Mn-sufficient enzyme achieved maximum activity of 105% of control at 2.0mM
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