Oxidation of carbon monoxide by Pseudomonas carboxydohydrogena
1983
Kim, Y.M. (Yonsei Univ., Seoul (Korea R.). Coll. of Sciences, Dept. of Biology ) | Hegemen, G. (Indiana Univ., Bloomington (U.S.A.). Dept. of Biology)
The stoichiometry between the consumption of CO and O2 and the production of CO2 (2CO + O2--2CO2) showed that Pseudomonas carboxydohydrogena grows as a typical aerobic CO oxidized with CO. The optimal concentration of CO for growth was found to be 30% in gas mixture with air. The reaction rate and stability were maximal at pH7.5, and the Arrhenius plot revealed an activation energy of 37.7 kJ/mol (9.0kcal/mol). The crude enzyme used methylene blue, thionin, and toluylene blue as electron acceptors for the oxidation of CO to CO2 under anaerobic conditions
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