Studies on protoplast isolation of Pleurotus cornucopiae
1986
Lee, Y.H. (Sookmyung Women's Univ., Seoul (Korea R.). Dept. of Biology) | Park, Y.H. | Yoo, Y.B. (Rural Development Administration, Suwon (Korea R.). Agricultural Sciences Inst.)
The optimal conditions for high yields of mycelial protoplasts from P. cornucopiae were established. The concentration of enzyme system containing Novozym 234, beta-D-glucanase and beta-glucuronidase was 5mg ml(-1) each. The osmotic stabilizer most effective for protoplast isolation was 0.6M sucrose. The optimal reaction time of mycelium with the lytic mixture was 90 min in a shaking condition at 120 strokes min(-1). When the mycelium of P. cornucopiae was cultured for 4 days on mushroom complete medium at 28 deg C, the formation of protoplast was effective
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