The protoplast fusion of Aspergillus niger
1986
Kim, M.S. | Choi, Y.K. (Hanyang Univ., Seoul (Korea R.). Dept. of Biology)
Conditions for production, fusion and reversion of protoplasts of Aspergillus niger were investigated, and an attempt was made to enhance fusion frequency. Auxotrophic mutants and morphological mutants were induced by U.V. irradiation (9.9 erg/mm2, 13 min) on Aspergillus niger. Maximum yield of protoplasts was obtained from 21 hr cultured mycelia by using 1% driselase in 0.6M KCL or 0.6M NH4CL as osmotic stabilizer. The optimal temperature for mycelium digestion was 30 deg C, and the optimal pH was 6.0
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