Feasibility of in vitro propagation of bananas and plantains
1985
Vuylsteke, D. (International Institute of Tropical Agriculture, Ibadan (Nigeria)) | De Langhe, E.
A method of rapid clonal propagation of bananas and plantains in vitro is described. Highly proliferative growth of adventitious buds is obtained by culturing pre-existing meristems on a modified Murashige and Skoog medium with high benzylaminopurine concentration. Regeneration of plantlets from these 'multiple buds' is achieved by subculturing on a medium with a low cytokinin content. Each multiplication stage is 8-10 weeks long; regeneration of plantlets ready for soil transplantation requires 3-4 months. The AAB and ABB genomes show the highest multiplication potential. The multiplication potential was more than 1 million meristem tips/explant/year. This in vitro technique is appropriate for rapid propagation and germplasm exchange
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