Axillary bud development from nodal cultures of bean seedlings (Phaseolus vulgaris L.)
1984
Martins, I.S. | Sondahl, M.R. (Institute of Agronomy, Campinas, SP (Brazil). Dept. Genetics)
The best conditions for the induction of development and growth of axillary buds of bean seedlings (Phaseolus vulgaris) consist of germination in the presence of 6-BA (20 uM) and culture of derived nodal regions in the basic B5 medium supplemented with 6-BA (5 uM) and IBA (0.25 uM). Rooting of the shoots derived from axillary buds was induced by IBA (10 uM) for 10 days, followed by culturing the plantlets in the basic B5 medium without growth regulators. The presence of light was essential to the development of axillary buds from nodal explants of bean seedlings. The frequency of axillary bud induction and the shoot growth were different according to the node position and genotype. The cultivar Puebla-153 presented higher induction frequencies, whereas better shoot growth was observed for the cultivar Carioca. The maximum number of buds developed per leaf axil was six (cv. Moruna, node I) suggesting a total of twelve dormant buds at each cotyledonary node of bean seedlings
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