Transfer of RP4:: Mu cts and RP4:: mini-Mu from E. coli to Pseudomonas sp.
1988
Ko, Y.W. | Huh, Y.J. | Lee, Y.N. (Korea Univ., Seoul (Korea R.). Dept. of Biology)
Chromosomal gene transferable hybrid plasmids, RP4::Mu cts and RP4::mini-Mu, were transferred by conjugation from E. coli to Pseudomonas strains. In order to use for recipient cells of RP4::Mu cts and RP4::mini-Mu, plasmid-free Pseudomonas strains were characterized for their antibiotic resistance, aromatic hydrocarbon utility and degradation patterns of chlorinated herbicide. Transfer frequencies of RP4::mini-Mu exhibited about 10** (-2) to 10** (-4), while those of RP4::Mu cts exhibited very low value of 10** (-7) in recipients tested except Pseudomonas aeruginosa KU557. Existence of hybrid plasmids in Pseudomonas transconjugants were identified by their antibiotic resistance and agarose gel electrophoresis. In case of RP4::Mu cts transconjugants it was also confirmed by demonstrating that they were capable of releasing phage and forming plaques 43deg C. Plaque forming unit of the transconjugants was about 10** (5). It was shown by the stability test that RP4::Mu cts and RP4::mini-Mu in Pseudomonas were relatively stable
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