Purification and cell wall regeneration of protoplasts from Pyricularia oryzae Cav.
1987
Han, S.S. | Lee, Y.H. | Yoo, J.D. | Lee, E.J. (Rural Development Administration, Suwon (Korea R.). Agricultural Sciences Inst.)
The optimum conditions for protoplast formation and regeneration from Pyricularia oryzae Cav. were selected as follows. As a basic solution, 0.02 M potassium phosphate buffer solution plus 0.6 M KCl adjusted to pH 5.2 with 1 N HCl was used. A mixture of enzyme combinations with 20 mg cellulase R-10/ml, 5mg macerozyme R-10/ml and 10 mg driselase/ml used as a lytic enzyme showed better lytic effect than any single enzyme treatment for protoplast formation. Two-day-old mycelia of P. oryzae grown in the mixture of three lytic enzyme solution at 30 deg C for 3 hr showed best condition for protoplasts formation. For regeneration from the protoplasts of P. oryzae, potato dextrose agar containing 0.02 M potassium phosphate plus 0.6M KCl used as a stabilizer was best for regeneration medium
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