Immunohistochemistry for detection of Aujeszky's disease virus antigens: Protein A-gold labelling of ultrathin sections for electron microscopy

Kim, S.B. . Coll. of Veterinary Medicine

Immunohistochemistry for detection of Aujeszky's disease virus antigens: Protein A-gold labelling of ultrathin sections for electron microscopy

1989

Kim, S.B. (Kyongsang National Univ., Chinju (Korea R.). Coll. of Veterinary Medicine)

The present study was carried out to determine viral antigens and its morphogenesis in the ultrathin frozen and araldite sections of cell cultures infected with ADV by protein A-gold labelling. ADV antigens were labelled with 10nm gold probes, and electron-dense gold particles were mainly present on viral nucleocapsids and viral envelopes. Immunogold labelling in the ultracryosections showed a very low degree of interaction with tissue structures. Immunogold labelling in the ultrathin cryosections can be useful tool for the detection of ADV antigens, and the technique also may provide its great potential for immunocytochemical studies on various virus-host cell interactions

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