Specificity of cell-mediated immunity in guinea pigs sensitized with Mycobacterium spp using the leukocyte adherence inhibition test
1989
Park, S.K. | Jun, M.H. | Lee, H.J. | Min, W.G. (Chungnam National Univ., Taejon (Korea R.). Coll. of Agricultuer) | Yoon, Y.D. (Rural Development Administration, Anyang (Korea R.). Veterinary Reserach Institute)
In order to measure in vitro cell-mediated immunity in the guinea pigs sensitized with the killed bacilli of Mycobacterium bovis (AN5), M avium (serotype 2), M tuberculosis and M intracellulare (serotype 8), leukocyte adherence inhibition (LAI) test was established using the antigens of purified protein derivatives (PPD) tuberculin. By using LAI test, specificity of cell-mediated immune responses of the guinea pigs inoculated with various Mycobacterium spp was investigated, and comparison between values of LAI and skin test was also made to evaluate the specificity of the newly designed test. The optimal concentration of PPD antigens for LAI test was 1 to 2mg per ml of medium. When the leukocytes of guinea pigs sensitized with both M bovis (AN5) and M avium (serotype 2) for 2 to 8 weeks were incubated with homologous or heterologous PPD antigens, mean values of LAI test were 61.2 +- 11.2 and 65.6 +- 5.1 % in homologous PPD antigens respectively, while 30.0 +- 3.7 and 32.8 +- 5.7 % in heterologous PPD antigens, showing the prominently high value of LAI in the homologous system (p0.01). When the leukocytes of guinea pigs sensitized with both M tuberculosis and M intracellulare (serotype 8) for 2 to 8 weeks were incubated with homologous and heterologous PPD antigens, mean values of LAI test were 67.9 +- 2.9 and 66.9 +- 5.0 % in homologous PPD antigens, while 27.4 +- 7.4 and 24.4 +- 7.1 % in heterologous PPD antigens, showing the prominently high value of LAI in the homologous system (p0.01). Comparing with the specificity of LAI and skin tests on the basis of the value obtained from the homologous system, deviation of reaction was revealed to be 49.5 to 100.2 in LAI test, and -15,9 to 52.0 in skin test
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