Saccharification of corn cobs with fungal enzymes
1989
Matanguihan, R.M. | Del Rosario, E.J. (Philippines Univ., Los Banos, College, Laguna (Philippines). National Inst. of Biotechnology and Applied Microbiology)
Cellulase and xylanase enzymes were produced in shake-flask and stirred-fermenter cultures containing 2% (w/v) ground corn cobs by Trichoderma reesei NRRL 11460 (T.r. 11460), Aspergillus niger NRRL 330 (A.n. 330), Penicillium funiculosum BCC 3116 (P.f. 3116) and T. harzianum 36. The prepared enzymes were used to saccharify corn cobs. The crude enzyme from P.f. 3116 gave the highest reducing sugar yield of 8.59 mg/ml and saccharification value of 17.2% based on initial weight of the dry substrate. An increase in saccharification was observed using mixed enzyme extracts from T.r. 11460 and P.f. 3116 at 3:7 ratio (v/v), as well as from T.r. 11460 and A.n. 330 at 7:3 ratio (v/v). The saccharification value using T.r. 11460 cellulase increased by about six-fold to 65.3% using corn cobs which had been pretreated with NaOH-Ethanol. The hydrolysate contained 32.7 mg/ml reducing sugars consisting of xylose glucose and cellobiose. T. reesei cellulase produced in cheese whey medium gave the highest saccharification values of 28.3 and 39.0% on corn cobs and Sigmacel, respectively. The same enzyme gave 97.6% saccharification of corn cobs which had been pretreated with a mixture of 1% NaOH and 50% ethanol at 121 deg C for 60 min.
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