Detection method of Aphelenchoides besseyi for routine examination of rice seedlots
1988
Gergon, E.B. | Mew, T.W. (International Rice Research Inst., Los Banos, Laguna (Philippines))
Different methods of detecting Aphelenchoides besseyi in rice grains were evaluated for use in routine seed analysis involving large number of seedlots. The highest number of A. besseyi was detected in 24 hours by manually separating the hulls from the kernel. This technique, however, is time-consuming. The other methods which used Baermann funnels or sieves in a dish gave lower detection rate but their use could be made feasible for routine examinations by extending the extraction period. Nematodes from pre-germinated or dry seeds may be collected after 48 to 72 hours to obtain a higher nematode recovery. The highest percentage infestation of 79.3% was obtained from deformed grains. The highest nematode count detected was 121 and the mean was 21 per seed.
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