Comparisons of serological and vector relationships among MAV-like isolates of barley yellow dwarf virus from Australia and the USA
1990
Lister, R.M. (Purdue University, W. Lafayette, IN (USA)) | Sward, R.J. (Plant Research Institute, Department of Agriculture and Rural Affairs, Burnley, Victoria (Australia))
Some barley yellow dwarf (BYD) isolates obtained from cereals collected in Victoria, Australia, during 1985-86, were serologically similar to the MAV isolate of W.F. Rochow, but seemed more akin to the PAV isolate of Rochow in vector relationships. Most serological comparisons used the Purdue culture of Rochow's MAV, initially obtained from Cornell but maintained by transfer by Sitobion avenae (Fabricius) during 6 years. However, detailed examination of the Purdue culture with monoclonal antibodies revealed that a change in its serological behavior had occurred during culture, although the characteristics of efficient transmission by S. avenae and not Rhopalosiphum padi (L.) had been maintained. This apparent change in serological behavior of the type MAV of Rochow during subculture was unexpected, and would not have been noted with the polyclonal antisera available to us, to which both the Rochow and Purdue cultures react similarly. Among possible reasons is selection from a mixture of MAV-like viruses having generally similar vector relationships. The results emphasize that, because aphid transmission is obligatory for transfer of BYDV, it is impossible to obtain "pure" cultures, for all BYDV isolates are likely to comprise mixtures of viruses that happen to be similarly transmissible by the vector(s) used for passage. In this way, the Australian MAV-like isolates, wich differ in vector relationships from the USA MAVs, may have been selected from introduced MAV-like infections by the aphid vectors that predominate in Australia.
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