The relationship between barley yellow dwarf virus content in aphids and their ability to transmit
1990
Barker, I. (Rothamsted Esperimental Station, Harpenden (UK)) | Torrance, L. (Ministry of Agriculture Fisheries and Food Agricultural Development and Advisory Service, Harpenden, Herts (UK))
Forecasting potential barley yellow dwarf virus (BYDV) outbreaks in the United Kingdom involves the daily trapping of cereal aphids and caging them individually on oat (Avena sativa L.) seedlings to estimate the percentage carrying virus. These plants cannot be diagnosed as infected much before 14 days after inoculation, and the direct testing of the aphids for virus presence might save this time and effort. Using an enzyme-amplified enzyme-linked immunosorbent assay (ELISA) technique, it was possible to detect an RPV-like BYDV isolate in single aphids and thus study the relationship between virus content in the aphid and its ability to transmit the virus to oat seedlings. Virus acquisition and retention in the aphid over time was also measured for the same RPV-like isolate comparing both efficient Rhopalosiphum padi (L.) and inefficient Sitobion avenae (Fabricius) vector aphid species.
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