Inhibition of nitrate reductase activity of microbial contaminants in hopped wort
1993
Bokvajova, A. | Demnerova, K. | Suchova, M. | Kralova, B. (Vysoka Skola Chemickotechnologicka, Prague (Czech Republic). Ustav Biochemie a Mikrobiologie) | Kral, M.
Two microbial strains with nitrate reductase activity, Hansenula sp. and Xanthomonas maltophilia were isolated from non-pasteurized hopped wort. The effect of five different inhibitors (riboflavin, L,D-tyrosin, ferric-ammonium citrate, niacin, nicotinamide, phenazinemethosulphate) on reduction of nitrate reductase activity (NRA), detected in microbial cells, was tested. The best inhibitory effect on NRA was obtained with riboflavin. Limiting inhibitory concentration of riboflavin was determined as 2.5 ppm. Other tested inhibitors applied in the same concentration partially reduced nitrate reductase activity of both microorganisms, too.
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