A simple method for isolating single fungal spores
1991
Huang, S.L. (Tottori Univ. (Japan). Faculty of Agriculture) | Kohmoto, K.
An improved method for single fungal spore isolation using a glass capillary was devised. Spore suspension was adjusted to a concentration (number of spores/ml) corresponding to the density that was theoretically calculated to contain only a single spore in a disgorded volume of the suspension from a glass capillary. The spore suspension was blotted on the sites of gellan gum or agar plates which were previously specified, and incubated to germinate vigorously for an appropriate period. The resulting germlings were examined for their singularity under a microscope. Monosporic colonies could be isolated easily and efficiently, without the aid of micromanipulator; the isolation rates were about 50% for Cephalosporium and 36% for Alternaria
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