The histochemical changes of mink skin with seasonal moulting
1991
Kondo, K. (Hokkaido Univ., Sapporo (Japan). Faculty of Agriculture) | Nishiumi, T. | Nakamura, F.
The histochemical changes of mink skin, particularly hair and hair follicle during the seasonal moult were studied using the staining methods of sudan, orcein, PAS, ferric ferricyanide and DACM. The results are summarized as follows: 1) The staining intensity of sebum that reacted with sudan in the sebaceous gland was constant throughout anagen and telogen, and the sebaceous gland secreted sebum actively even in telogen. 2) The number of elastic fibers stained with orcein was also constant throughout anagen and telogen. The features shown when using the orcein stain suggested that the elastic fibers played a role in anchoring the hair follicle. 3) PAS positive glycogen was observed only at the outer root sheath between sebaceous gland and hair bulb in anagen. 4) The keratinizing zone of hair in anagen, stained with ferric ferricyanide, was the same as PAS positive zone, but the hair in telogen was negative to ferric ferricyanide. Keratinization progressed from the hair cuticle at hair bulb cervix to the hair cortex below the sebaceous gland. 5) The localization of sulfhydryl groups and disulfide bonds detected by DACM suggested the keratinizing process of hair observed in ferric ferricyanide staining. DACM staining showed the existence of sulfhydryl groups in hair in telogen and the keratinization of hair follicles
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