Genome mapping of Magnaporthe grisea
1994
Hayashi, N. | Naito, H. (National Agriculture Research Center, Tsukuba, Ibaraki 305 (Japan))
A restriction fragment length polymorphism (RFLP) map has been constructed for the nuclear genome of the plant pathogenic ascomycete Magnaporthe grisea (anamorph = Pyricularia oryzae and Pyricularia grisea). The segregation of 61 RFLP markers, five host-specificity gene loci and the mating type locus was analyzed among 82 randomly selected colonies from a cross between parental strains CHNOS 37-1-1 and CHNOS 32-2-4, which are field isolates and pathogenic on rice (126.0 and 017.5 in the race code based on Japanese differential rice varieties, respectively). The present map consists of thirteen linkage groups. These linkages totalled 372 centimorgans (cM). One RFLP marker appears to be closely linked to the putative avirulence gene Avrl-TA which functions specifically to prevent strains of rice blast pathogen from infecting rice cultivar Tsuyuake. Whole chromosomes from the parental fungus strains were separated using contour-clamped homogeneous electric field (CHEF) electrophoresis. Six chromosomes were identified; one of the bands appears to be a doubled band. The estimated size of these bands, based on migration rates relative to those of chromosomal DNA of Schizosaccharomyces pombe, is from 1.8 to more than 10 megabases (Mb). The total genome size was estimated to be roughly from 34 to 38 Mb
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