Plant regeneration from cultured fertilized ovules and immature embryos of Triticum aestivum L.
1993
Ponitka, A. | Slusarkiewicz-Jarzina, A. (Polish Academy of Sciences, Poznan (Poland). Inst. of Plant Genetics)
In intergeneric and interspecific crossing there frequently occurs inhibition in the development of hybrid proembryos as a result of endosperm degeneration. Hence the necessity of in vitro culture of immature embryos. The paper presents attempts to obtain wheat plants through the culture of ovules isolated 1-4 days after pollination and 5-9 day embryos. The medium B sub (5) without 2,4-D was applied. Plants were obtained from the ovules and 7-9 day embryos, whereas 5-6 day embryos gave no plants. All obtained plants developed directly from embryos; no callus formation was observed
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