Studies on the in vitro propagation of Mussaenda erythrophylla "Rosea"
1991
Brookner, N.
Investigations to determine a suitable medium for the in vitro propagation of Mussaenda revealed that of the macro-element formulations, MS appeared to be the most suitable for the growth and multiplication of shoots. 6-BAP enhanced the elongation of stage I buds and promoted shoot proliferation. Cytokinin in stage I may be inessential since shoots formed in primary medium containing GA3 produced an acceptable rate of proliferation in stage II. Elongation of buds was significantly increased at 31 deg. C. Shoots produced in nodes at positions 2 and 3 were significantly longer than those in other positions. Multiplication rate was less in shoots from nodes at position 1. Shoots did not produce roots in vitro, rooting extra vitrum appeared to be more promising. Vitreous shoot formation was promoted at sucrose levels of 50 g/l, or when the concentration of ammonium nitrate in MS salts was reduced to one third. Phloroglucinol in culture medium reduced the advent of vitrescence.
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