Calcium sequestration in ischaemic rat brain
1994
Jalc, P. (Slovenska Akademia Vied, Kosice (Slovakia). Ustav Neurobiologie)
Adult normothermic rats were subjected to 30 min cerebrocirculatory arrest followed by blood recirculation for 24 h. At the end of the recirculation period, calcium (Ca) sequestration in the cerebral cortex and hippocampus was measured and compared with ultrastructural damage as assessed by electron microscopic cytochemistry. In animals without post-ischaemic hyperoxia, neither Ca content nor mitochondrial Ca sequestration were significantly increased in either the cerebral cortex or hippocampal subfield CA1. Calculation of tissue swelling revealed no change in the cerebral cortex but did reveal a volume increase in hippocampus, indicating development of brain oedema in this region. In animals with post-ischaemic hyperoxia tissue calcium significantly increased both in cortex and hippocampus. Calculation of tissue swelling revealed a volume increase also in cortex and hippocampus. The results do not support the hypothesis that selective vulnerability of hippocampus is the consequence of neuronal Ca overload, but rather indicate that Ca accumulation is an unspecific epiphenomenon of irreversible cell injury.
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