Effect of gamma irradiation on Amorphophallus kerrii NE. Brown tissue culture
1989
Tanya Khumlert
Survival of calli decreased as rate of gamma radiation increased and they could not survive after exposion to gamma radiation higher than 0.5 krad. Survival of these calli depended on their ages. Growth and development of calli decreased as doses of gamma radiation increased as well. All callus cultures were dead when exposed to 1120 rad of gamma radiation. The LD30 of callus grown on petiole segment and leaf segment were 672, 704 rad respectively. But LD50 of calli grown on both petiole and leaf segments were 800 rad. Leaf number and petiole length of plants regenerated from gamma radiation exposed calli were reduced when dose of radiation increased. Uncommon morphological characteristic of the first leaf such as fused leaf margin, linear leaf, torn leaf and mottled leaf was found, but disappeared on the third and forth leaves. Gamma radiation had no effect on color of corm and size of stomata.
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