New primers for the polymerase chain amplification of cpDNA intergenic spacers in Phaseolus phylogeny
1997
Fofana, B. | Harvengt, L. | Jardin, P. du (Faculte Universitaire des Sciences Agronomiques de Gembloux (Belgium). Unite de Biologie Vegetale) | Baudoin, J.P.
A better knowledge of the phylogenetic relationships among agricultural and forestry plant species is of a great importance in plant genetic resource management. The conservative mode of chloroplast DNA (cpDNA) evolution suggests that any change in structure or content of the chloroplast genome may have significant phylogenetic implications. We describe in this paper six new PCR primers designed for the amplification of three cpDNA intergenic spacers: atpB-rbcL, rps14-psaB, and petA-psbE. The target regions were amplified in various botanical families belonging to both the monocotyledon and dicotyledon classes, indicating the wide interest of the primers for taxonomic studies in angiosperms. In Leguminosae, the digestion of amplified regions by four- and five cutter enzymes revealed cleavage site variation at the intergeneric, interspecific and even at the intraspecific level. Phylogenetic implications of this polymorphism are being established in Phaseolus species.
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