Somatic embryogenesis and efficient plant regeneration in immature inflorescence culture of Setaria italica (L.) Beauv.
1996
Vishnoi, R.K. | Kothari, S.L. (University of Rajasthan, Jaipur (India). Dep. of Botany)
Immature inflorescences of Setaria italica (Fox-tail millet) var. Arjuna varying in length from 0.5-2.0 cm were cultured on MS medium supplemented with 2.0 mg/l 2,4-D (2,4-Dichlorophenoxyacetic Acid), PIC (Picloram) or NAA (alpha-Naphthaleneacetic Acid) in combination with 0.5 mg/l Kn (Kinetin). Both non-embryogenic types of callus formation were observed depending upon the size of the explant and the growth hormone present in the culture medium. Formation of embryoids was also observed on embryogenic callus. On transfer to hormone free MS medium, calli gave rise to plantlets at varying frequency. Explants cultured on NAA containing medium developed a few shoots from the axil of bracts in the primary cultures, which further elongated on transfer to hormone free MS medium
Показать больше [+] Меньше [-]