Phylogenetic relationship of Caprini estimated by cytochrome b gene sequence analysis
1997
Arai, K. (Ibaraki Univ., Ami (Japan). Faculty of Agriculture) | Munechika, I. | Ito, I. | Kikkawa, A. | Nakamura, K. | Kanazawa, T. | Kosugiyama, M.
Phylogenetic relationship of three wild species and two livestock species of Caprini was estimated based on nucleotide sequences for cytochrome b gene in mitochondrial DNA. DNA samples were prepared from blood and liver of Pasang (Capra aegagrus), Markhor (Capra falconeri), Mouflon (Ovis musimon), domestic goat (Capra hircus) and domestic sheep (Ovis aries), respectively, and used for amplifying whole cytochrome b gene DNA by the polymerase chain reaction. The base sequences were directly determined by the dideoxy terminator cycle sequence method using the amplified DNA. In the cytochrome b gene (1140 bp), maximal homologies, 99.4% and 99.3%, were obtained between Pasang and domestic goat, and between Mouflon and domestic sheep, respectively. The next closest combinations were between Markhor and domestic goat (96.6%) and between Pasang and Markhor (96.0%). Any of the other combinations gave lower homologies. The phylogenetic tree constructed by the neighbor-joining method from the present and previous results confirmed that Pasang and Mouflon as closest to domestic goat and domestic sheep, respectively, though Markhor had also closer relationship to domestic goat and Pasang than to Mouflon and domestic sheep. These results strongly support the previous hypothesis from morphological studies that domestic goat domestic sheep have been established from Pasang and Mouflon, respectively, and Markhor has contributed partially to the establishment of domestic goat
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