Multiple shoot differentiation from excised shoot tips and nodal sections of maize (Zea mays L.)
1998
Perez, E.A. | Dimaculangan, J.G. | Sasuman, L.A. | Damasco, O.P. (Philippines Univ. Los Banos, College, Laguna (Philippines). Inst. of Plant Breeding)
In vivo techniques have been developed to regenerate clumps of multiple shoots at high frequency from excised shoot tips and nodal sections of in vivo germinated seedlings of maize (Zea mays L). Each shoot tip produced about 7 shoots after 4 weeks of culture on MS medium supplemented with 2 mg/L BAP, 500 mg/L casein hydrolysate and 20 g/L sucrose. Clumps of very small shoots were also produced from the nodal sections. Shoots developed into plantlets and formed roots when transferred onto MS medium supplemented with 1 mg/L IBA. Shoot tip cultures of 9 inbred lines (8 yellow corn and 1 white corn) formed multiple shoots, with frequency ranging from 5 to 100 percent. Inbred line P22 gave the best multiple shoot response. This technique of axillary/adventitious shoot differentiation offers an efficient and high frequency regeneration system suitable for maize genetic transformation work
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