Probe for differentiation of Xanthomonas oryzae pv. oryzicola and X. oryzae pv. oryzae
1996
Raymundo, A.K. | Perez, M.T.M. | Leach, J.E. (Philippines Univ. Los Banos, College, Laguna (Philippines))
A previously cloned amplification product generated from Xanthomonas oryzae pv. oryzicola by primers based on the upstream region of the promoter for the hrpX00 (hypersensitivity and pathogenicity) gene was used as probe to compare the genomes of X. oryzae pv. oryzicola and pv. oryzae. The clone designated as pHrp256, hybridized to a 9.4 kb BamHl fragment in different isolates of the bacterial leaf streak pathogen, X. oryzae pv. oryzicola. Likewise, a 5.4 kb BamHl fragment was detected in isolates of the closely-related rice pathogen, X. oryzae pv. oryzae. Therefore, DNA from the two microorganisms contain a size polymorphism in the BamHI fragment containing a region of the hrpXoo promoter. The size polymorphism can be used to differentiate the two microorganisms
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