Factors affecting a laboratory bioassay for antibiosis: Influences of maize silks on the corn earworm and fall armyworm larvae
1997
Wiseman, B.R.
A useful laboratory bioassay has been developed to screen for resistance to lepidopterous insects attacking maize, Zea mays L., And over use in studying the antibiotic mechanism and bases of resistance to these insects. The bioassay may be used to detect minor as well as major differences between the resistant and susceptible maize cultivars. The bioassay has been used to study the influence of: the environment; pollinated vs. nonpollinated silks; ear position; age and type of silk; and callus tissue on expressions of antibiosis against the corn earworm (CEW), Helicoverpa zea (Boddie), or fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith), larvae. Studies on some of the factors, such as temperature, diet and diet ingredients, and insect feeding responses, revealed interactions with the expressions of antibiosis. The bioassay has also been used in studies on the relationship between low larval weight with maysin content and the genetic and chemical bases of resistance in maize to CEW and FAW larvae.
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