Sensitive enzyme-linked immunosorbent assay for screening aflatoxin B1 in food and feedstuffs
1997
Barna-Vetro, I. | Solti, L. | Szabo, E. (Institute for Animal Sciences, Godollo (Hungary). Agricultural Biotechnology Center)
Aflatoxins are toxic metabolites produced by strains of Aspergillus flavus and A. parasiticus. They occur worldwide and contaminate certain foods and feedstuffs, especially wheat, corn, soya and groundnut products. Although the climate in Hungary is not favourable for production of these moulds, by consumption of imported foods these mycotoxins can cause serious health problems. As aflatoxin B1 is highly carcinogenic, its accepted level is regulated in many countries; the official limit in Hungary is 5 ng per g. The application of a suitable analytical method for detection and quantification of aflatoxin B1 is very important. Physico-chemical methods (TLC, HPLC, GC-M) and measuring mycotoxins are time-consuming and quite expensive. Five years ago our institute started a research program for developing ELISA tests to detect mycotoxins in food and feeds, resulting in reagent kits for T-2, zearalenone and ochratoxin-A. This program continued with the development of sensitive, monoclonal antibody-based direct, competitive ELISA test and reagent kit for quantitative measurement of aflatoxin B1 with a detection limit of 0.9 ng per g in cereals. The meat within-assay and interassay coefficients of variation of the standard curves were less than 10 percent. With acetonitrile-based extraction solvent the meat recovery values from cereals contaminated artificially with this mycotoxin was about 80 percent
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