Contribution to the method of grapevine propagation by microcuttings in vitro
1998
Dimku, M. | Pandeliev, S. (Vissh Selskostopansk Institut, Plovdiv (Bulgaria))
In period 1996-1997, investigations were carried out with the Ferkal rootstock and the grapevine cultivar Cabernet Sauvignon on the development of single bud microcuttings in different sized glass containers in vitro and the shoot growth during adaptation in perlite. It was established that the conditions in the 500ml glass banks were much more favourable for the single bud cutting development than those in test tubes and small 250ml glass banks. The plant cultivation in the former saved time for washing, nutrient loading and transportation when needed, which led to a 17 leva reduction in the prime cost per plant. The plant development energy created in the growth chamber exerted a positive after effect during the period of adaptation. The plants passed through the second stage of development for a shorter period of time and developed more rapidly their first leaf than those grown in test tubes. The 500ml glass banks created better conditions for reusing the micropropagation plants which led to increase in the coefficient of propagation.
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