Optimization of omega-3 fatty acids from marine microalgae
1996
Penkhae Anankusri
Twelve marine microalgae were screened for their capacity to produce polyunsaturated fatty acids, such as eicosapentaenoic acid. (EPA). Chaetoceros calcitrans was selected for optimal EPA production. Studies on the effects of physical factor and nutrient composition on growth and EPA production by C. calcitrans were conducted. Temperature, initial pH, aeration and photon flux density were highly influenced growth and EPA production. Highest EPA production was obtained at the following conditions: temperature at 22 deg C., initial pH at 9.0., aeration rate at 80 cubic cm/min supplemented with 2-3 percent CO2 and photon flux density at 200 micro mole/square m/s. Effects of nutrient composition was studied on nitrate, phosphate, sodium chloride, silicate, bicarbonate and vitamin B1, B12 in culture medium. Nitrate concentration at 150 mg/l, phosphate at 20 mg/l and sodium chloride concentration at 25-30 g/l were optimal concentration for growth and EPA production. While silicate concentration at 40 mg/l supported maximal growth and highest EPA production. Bicarbonate at concentration of 50 mg/l did not affect growth but promoted EPA production consideration. This strain showed little requirement of both vitamins for growth and EPA production. Comparison of kinetic growth of C. calcitrans on Modified Walne's medium and the original formular indicated that the former had increased specific growth rate to 0.9 d and decreased doubling time to 1.05 d. Study on distribution of lipid classes of C. calcitrans revealed that main lipid classes were triacylglycerol (TG), mono-galactosyldiacylglycerol (MGDG) and di-galactosyldiacylglycerol (DGDG).
Показать больше [+] Меньше [-]Ключевые слова АГРОВОК
Библиографическая информация
Эту запись предоставил Kasetsart University