Role of water content and incubation time in measuring phosphate activity in soils
1998
Salam, A.K. (Universitas Lampung, Bandar Lampung (Indonesia). Fakultas Pertanian)
Activities of enzymes in soils are affected by soil moisture contents, thus water content of soil samples is very important in determination of soil enzymatic activities. This research was to evaluate the effects of water content and incubation time in measurement of phosphatase activity in soils. Soil samples were an Ultisol from Aichi Prefecture, Japan, and 4 different soils (Ultisol, Oxisol, Andisol and Alfisol) from Lampung, Indonesia. The Japanese soil sample was treated in laboratory with water at 0 to 120 percent (water-to-soil ratios 0 to 1.2) and incubated for the time ranging from 0 to 15 days before enzyme analysis while the Indonesian soil samples were incubated at a water-to-soil ratio of 0.4 for 0 to 12 weeks. Activity of phosphatase in the Japanese soil increased significantly with water content and reached a maximum value at water-to-soil ratio of 0.4 and decreased at higher water contents. Incubation time increased the phosphatase activity showing the first order of kinetics
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