Ultrastructure of chloroplast in the young leaves of satsuma mandarin top sprayed with different fertilizers under plastic house conditions
1998
Aguja, S.E. | Shiraishi, M. (Ehima Univ., 3-5-7 Tarumi, Matsuyama 790 (Japan). United Graduate School of Agricultural Sciences)
The ultrastructure of chloroplasts, starch grains, thylakoids and grana of 15-day-old leaves of summer shoots of `Okitsu Wase' satsuma mandarin (Citrus unshiu Marc. cv. Okitsu Wase) trees top sprayed with different foliar fertilizers were studied under plastic house conditions. The upper and lower epidermis in all treatments were composed of small rectangular cells while the mesophyll consisted of an undifferentiated palisade and spongy cells. The no-fertilizer control and Liquid Fertilizer-B (LF-B; 0-6-4) treated leaves possessed bigger and spherical chloroplasts while urea (46-0-0), Liquid Fertilizer-A (LF-A; 10-3-4) and Liquid Fertilizer-C (LF-C; 1-6-4) treated leaves formed small and ellipsoidal to elongated ones. The number and size of starch grains in the chloroplasts decreased but the number of thylakoids increased with the application of nitrogenous fertilizers such as urea, LF-A and LF-C. Grana formation was promoted by foliar sprays of LF-A and LF-C, fertilizers that contain low nitrogen levels, whereas the non-nitrogenous fertilizer, LF-B resulted in less formation of grana
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