Effect of bacterial-enzymatic additives on fermentation process and fibre composition in silaging grass matter

Trials were carried out to verify the effectiveness of biological additives in silaging grass matter. Non-treated grass was silaged in control variants, faded to a lower content of dry matter, 433.9 g/kg (variant A) and a higher content of dry matter, 479.8 g/kg (variant F). In the positive control, faded matter was treated by formic acid (variant B). In variant C was applied the additive comprising an inoculant (S. diaccetylactis, S. cremoris, L. plantarum, L. acidophilus) with enzymes (celulases, amylases, proteases), in variant D an inoculant (L. plantarum, S. faeculum, P. acidilacti) with enzymes (celulases, amylases, pantozanases) and in variant E an enzymatic additive (celulases, hemicelulases, glucozooxidases). During the silage fermentation, the content of reducing sacharides was significantly decreased, especially in variant D (P0.001), whereas the lowest change was detected in variant B with chemical conservant. The silage of control variant showed the highest amount of lactic acid. In the case of the silage with enzymes, the highest content of lactic acid was found in variant D, and there were significant differences when compared with variants B, C and F. The silage with biological additives contained a higher amount of acetic acid over non-treated variants, with significant differences between C:F, D:F and E:F. As for the fibre fractions, ADF content was not significantly higher in the silage of variants A, C, D, E in comparison with grass matter, and a fall was found in variants B and F. The content of NDF was significantly reduced in all variants of silage, this decrease being correlated with a significant reduction in the content of hemicelulose. The most significant decrease (P0.001) in hemicelulose content was observed for the silage with biological additives. The content of celulose significantly declined in variant B as compared to the grass matter and to silages of variants A and E. Celulase degradation was due to strong chemical conservant. The treatment by enzymes of grass matter had no significant effect on the results of the fermentation process. Partial degradation of plant cell wall components did not cause a significant increase in the disponible source of yested sugars