Distribution and molecular characterization of tomato yellow leaf curl virus in tomato (Lycopersicon esculentum) and pepper (Capsicum sp.) in Jamaica
1997
Wernecke, M.E. (West Indies Univ., Mona (Jamaica). The Biotechnology Centre)
Since 1991, farmers in southern and central Jamaica have reported losses of up to 100 percent in their tomato and pepper crops. A concurrent increase in whitefly population and symptoms of leaf curling and yellowing lead to the presumption that the causal agent of "Jherri Curl" disease was a whitefly-transmitted geminivirus. Polymerase Chain Reaction (PCR) primers specific for tomato yellow leaf curl virus (TYLCV-Is) were used to amplify full-length as well as subgenomic viral fragments from total DNA extracted from tomato (Lycopersicon esculentum) with "Jherri Curl" symptoms and pepper (Capsicum sp.) with viral symptoms. These viral PCR fragments were cloned from tomato plants collected from 3 areas and from a pepper plant collected in St. Elizabeth. Nucleotide sequence comparison of these three clones from tomato and one from pepper gave 97 percent identity to TYLCV-Is. Nucleotide sequence identities of the intergenic regions among the four viral clones were 99 percent. These results indicate that TYLCV associated with these tomato and pepper plants were all isolates of TYLCV-Is.... Electric discharge particle acceleration inoculation of tomato and pepper using the full-length clone from tomato was used to confirm infectivity of the Jamaican isolate of TYLCV-Is on tomato and pepper. This is the first report showing the association of TYLCV with pepper in Jamaica.
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