An analysis of yam anthracnose by isolation and partial characterisation of phytotoxins of Colletotrichum gloeosporioides and tissue culture of Dioscorea alata (yam)
1997
Alleyne, A.T. (West Indies Univ., Cave Hill (Barbados). Dept. of Biological and Chemical Sciences)
Ninety-seven isolates of Colletotrichum gloeosporioides were obtained from diseased leaves of yam, anthurium, avocado, mango, orange, grapefruit, and coffee. Two from yam and one each from anthurium and grapefruit were used to extract phytotoxins from liquid cultures. These phytotoxins were partially purified by gel filtration chromatography and SDS-polyacrylamide gel electrophoresis, and analysed for protein and or carbohydrate by colorimetric assays and gas chromatography. Preliminary results suggest that phytotoxins produced by isolates of C. gloeosporioides are composed of carbohydrates ranging from approximately 4-5 kDa in size. These are linked to proteins also approximately 4 kDa in size....
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