Fast analytical method for pollution detection based on the ATP hydrolysis catalysed by ATPase
1997
Vujisic, Lj. (Institut za nuklearne nauke Vinca, Beograd (Yugoslavia). Laboratorija za fizicku hemiju) | Vasic, V. | Krstic, D. | Jovanovic, D. | Horvat, A. | Nikezic, G.
Spectrophotometric method for determination of inorganic phosphate liberated in hydrolysis of ATP catalyzed by ATPase was modified in order to obtain faster procedure, which could also be used in none laboratory conditions. The modification has some advantages compared to the most used Pennial method: a) the reagents are stable for several months; b) the 45 min procedure of phosphomolibdate extraction by isobutanole-benzene is ommited, and the method is not dangerous for the analyst; c) color develops after 20 min. The method was tested on the determination of inorganic phosphate in the presence of cadmium nitrate as inhibitor of ATPase activity. The results were compared to the results obtained by Pennial method. The results obtained have shown some good agreements.
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