The use of growth factors and messengers of their action for improvement of maturation of porcine oocytes
2000
Sirotkin, A. (Research Institute of Animal Production, Nitra (Slovakia)) | Dukesova, J. | Makarevich, A. | Kubek, A.
The aim of the in-vitro experiments was to study the effect of growth factors (insulin-like growth factor-I (IGF-I), insulin-like growth factor-II (IGF-II) and epidermal growth factor (EGF), regulators of protein kinase A (PKA) on in-vitro nuclear maturation and protein kinase A level in porcine oocyte-cumulus complexes. It was found that addition of IGF-I, IGF-II or EGF, or combination of IGF-I and EGF, significantly increased the proportion of oocytes which reinitiated and completed meiosis, as well as the content of protein kinase A within the oocytes (proportion of cells containing catalytic and, in some cases, regulatory subunits of protein kinase A). Inhibitors of protein kinase A, when given alone, did not influence substantially the proportion of oocytes which reinitiated or completed meiosis. However, Rp-cAMPS and KT5720 both prevented the stimulatory effects of IGF-I, IGF-II and EGF on the reinitiation and completion of oocyte maturation. The present observations suggest that IGF-I, IGF-II and EGF are potent stimulators of oocyte nuclear maturation. The action of growth factors on oocytes are mediated through protein kinase A-dependent intracellular mechanisms.
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