Assay of antibiotic detection limits in cow's milk model samples and comparison of sensitivity of various detection systems (disk diffusion method, Delvotest SP and Penzym S 100)
Hozova, B. | Kratmuellerova, M. (Slovenska Technicka Univ., Bratislava (Slovak Republic). Chemickotechnologicka Fakulta)
The work was aimed at estimating the detection limits of 21 antibiotics (beta-lactam antibiotics, cephalosporins, aminoglycosides, macrolides and others) by means of three types of detection systems (disk diffusion method with Bacillus stearothermophilus var. calidolactis C 953, Delvotest SP and Penzym S 100) and to verify their sensitivity in the evaluation of the admissible maximum residual limits (MRL). The high sensitivity and the good correlation of results have been achieved mainly by applying the rapid methods, i.e. Delvotest SP and Penzym S 100, versus the less sensitive disk diffusion method. In the next stage of the work, detection limits of the mutual combinations of antibiotics in milk were estimated. In a model experiment, synergic effects between ampicillin and oxacillin, cefuroxime and trimethoprim and between cephalosporins (combination of cefazolin with cefoperhazon) were observed.
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