Use of anthocyanin biosynthesis stimulatory genes as markers for the genetic transformation of haploid embryos and isolated microspores in wheat
1999
Mentewab,A. | Letellier,V. | Marque,C. | Sarrafi,A.
Experiments were undertaken to study the use of anthocyanin biosynthesis stimulatory genes under the control of CaMV35S promoter and first intron of maize alcohol dehydrogenase (Adh1), as marker genes for genetic transformation of wheat haploid embryos and microspores by microprojectile bombardment.Transient expression was monitored for isolated microspore cultures of 1 and 8 days, after bombardment at 650 and 1100 psi.Anthocyanin expression in 1 day cultured microspores was only recovered at 650 psi, while a higher expression efficiency was obtained with 8 days old microspore culture at 1100 psi.Experiments conducted on embryos show that total regeneration as well as albino and gren plant regeneration of embryos were not affected by bombardment.64.5% embryos presented spots and 6.2% of the regenerated plants were chimeric, with sectorial expression of anthocyanin on leaves.
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