Detection of cross-reacting virus-specific human and animal antibodies
1999
Rusvai,E. | Szesztay,¦. | McLena,S. | Brojnas,J. | Koczan,A. | Visontai,I. | Gyuris,¦. | Gyïri,Z. | Egyed,L. | Herczeg,J. | Pálfi,V. | Lomniczi,B. | Minárovits,J. | Berencsi,Gy.
A long series of human and animal viruses possess cross-reacting antigens e.g. adenoviruses, pestiviruses, immuno-deficiency viruses and herpesviruses.In many cases, clinical diagnostics of viral zoonoses require use of the same antigen preparations for the detection of antibodies in different animal species and humans.Staphylococcus A- and Streptococcus G-peroxidase conjugates have been used for the detection of antibodies by biochemist and histologists since many years.This work describes the adaptation of Fc-specific conjugates for the detection of virus-specific human and animal antibodies using commercial ELISA reagents and IF antigen preparations.Recombinant HCV,HEV and HIV enzyme immuno assays were used throughout.The Epstein-Barr virus antigen was prepared according to standard diagnostic immuno-fluorescence procedures.
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