Optimizing the culture conditions for higher inulinase production by Kluyveromyces sp. Y-85 and scaling-up fermentation
1998
Wei, W. (Xiamen Univ. (China)) | Zheng, Z. | Liu, Y. | Zhu, X.
The response surface method (RSM) was used to optimize the medium for the production of inulinase by Kluyveromyces sp. Y-85. The inulinase production was adequately approximated with a full quadratic equation obtained from a four-factor-five-level central composite design. Analyses of the quandratic surfaces showed that in a 24-h fermentation at 30 degrees C, the maximum inulinase activity 59.5 U/ml appeared at extract of Jerusalem artichoke, urea, beef extract, corn steep liquor concentrations 8.0, 2.0, 0.2 and 4.0%, respectively. We further investigated the fermentation in 15 l fermentor and scaling-up in 1000 l tower fermentor. The inulinase activity in the scaling-up was 68.9 U/ml, which was the highest production reported at present, indicating that Kluyveromyces sp. Y-85 was an excellent strain for industrial production
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