Changes in the level of protein and activity of Cu/Zn-superoxide dismutase in zinc deficient rice plant, Oryza sativa L
1999
Obata, H. (Mie Univ., Tsu (Japan). Faculty of Bioresources) | Kawamura, S. | Senoo, K. | Tanaka, A.
In order to clarify the physiological role of Zn in rice plants through the identification and characterization of proteins affected by Zn deficiency, changes in the peptide profile in Zn-sufficient and Zn-deficient rice meristems were examined by two-dimensional pI-SDS PAGE. The level of some peptides fluctuated under Zn deficiency. One of the peptides for which the spot intensity decreased in the profile derived from Zn-deficient rice meristems was identified as Cu/Zn superoxide dismutase (Cu/Zn-SOD) by amino-terminal sequencing. Decrease in the Cu/Zn-SOD activity and of the Cu/Zn-SOD protein content was observed based on a nitro blue tetrazolium reduction assay and immunoblotting analysis, respectively. These results indicate that Zn deficiency in rice causes a reduction of the activity of Cu/Zn-SOD through the decrease of the protein content and/or inactivation of the enzyme in the meristem
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