Determination of imazalil in citrus fruits by HPLC using derivative analysis of spectra
1998
Ohura, T. (Toyama-ken. Inst. of Health (Japan)) | Yamamoto, A. | Kodama, S. | Matsunaga, A.
A simple and rapid method for determination of imazalil in citrus fruit was developed by HPLC using derivative analysis of spectra. Imazalil was extracted from citrus fruit with acetonitrile under alkaline conditions and the extract was concentrated to dryness. The residue was dissolved in 10 mmol/L KH2PO4-acetonitrile (2 : 3) and filtered through a 0.45 mum membrane filter. The sample solution thus prepared was subjected to HPLC with photodiodearray (PDA) detection. The first to forth-order derivative spectra, that have characteristic maxima and minima, were calculated from imazalil spectra data recorded with the PDA. By plotting the first to fourth-order derivative values at the above maxima and minima from sample spectral data against analysis time, several derivative spectrum chromatograms were obtained. We found that imazalil in samples could be determined from only third-order derivative spectrum chromatograms at 213 and 233 nm. The recoveries of imazalil in citrus fruits fortified at 1 mug/g and 4 mug/g were 89.8 to 90.1 % and 91.9 to 93.3 %, respectively. The proposed method was applied to the analysis of commercially available citrus fruits. Imazalil contents thus determined agreed well with these obtained by the official method. The lower limit of determination of imazalil in citrus fruit was found to be 0.5 mug/g
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